须鳗虾虎鱼线粒体全基因组测序和系统发育分析  

Complete mitochondrial genome sequencing and phylogeny analysis of goby(Taenioides cirratus)

作　　者：梁阳阳 张国庆 陈诚 赵秀侠[1] 高娜 李静[1] 方婷[1] 杨坤[1] 尹峰 郭伟 卢文轩[1] LIANG Yangyang;ZHANG Guoqing;CHEN Cheng;ZHAO Xiuxia;GAO Na;LI Jing;FANG Ting;YANG Kun;YIN Feng;GUO Wei;LU Wenxuan(Key Laboratory of Freshwater Aquaculture and Enhancement of Anhui Province,Fisheries Research Institute,Anhui Academy of Agricultural Sciences,Hefei 230001,China;Fishery Management Station,Chaohu Administration,Hefei 238001,China)

机构地区：[1]安徽省农业科学院水产研究所水产增养殖安徽省重点实验室,安徽合肥230001 [2]巢湖管理局渔政管理总站,安徽合肥238001

出　　处：《大连海洋大学学报》2023年第5期772-778,共7页Journal of Dalian Ocean University

基　　金：安徽省自然科学基金(2008085QC105);财政部和农业农村部:国家现代农业产业技术体系资助(CARS-46);安徽省水产产业技术体系(皖农科函[2021]711号);安徽省农业科学院湿地生态与应用技术创新团队项目(2021YL055)。

摘　　要：为了解须鳗虾虎鱼(Taenioides cirratus)线粒体基因组特征和系统发育状况,采用Illumina Hiseq高通量测序技术,测定了从南渡江口、珠江口、太湖、巢湖和南四湖中采集的12尾须鳗虾虎鱼的线粒体全基因组,并下载近缘种线粒体全基因组序列进行系统发育分析。结果表明:须鳗虾虎鱼线粒体全基因组长为16641~16978 bp,包含13个蛋白编码基因、22个tRNA基因、2个rRNA基因和1个D-loop控制区,各样本的编码基因序列长度较一致,线粒体全基因组长度差异主要是由控制区长度(975~1314 bp)差异造成的;基于13个蛋白编码基因和2个rRNA基因序列构建系统发育树,须鳗虾虎鱼分为3个高支持率进化枝,与红狼牙虾虎鱼(Odontamblyopus rubicundus)、鲡形鳗虾虎鱼(Taenioides anguillaris)形成并系,太湖、巢湖和南四湖的须鳗虾虎鱼个体聚为1个进化枝,南渡江口和珠江口的须鳗虾虎鱼个体均分属2个进化枝。研究表明,青藏高原隆起和亚洲季风形成带来的中国水系格局变化,可能是须鳗虾虎鱼形成目前系统发育结构的重要原因,本研究结果可为进一步明确须鳗虾虎鱼的分类地位和资源保护提供重要依据。To clarify the mitochondrial genome characteristics and phylogenetic status of goby(Taenioides cirratus),the complete mitochondrial genomes were analyzed in 12 samples collected from the Nandu River Estuary,Pearl River Estuary,Taihu Lake,Chaohu Lake and Nansi Lake and download the mitochondrial genome sequences of closely related species for phylogenetic analysis by the second generation high throughput sequencing technology.Results showed that the length of individual mitochondrial genome was ranged from 16641 to 16978 bp,containing 13 protein coding genes(PCGs),22 tRNA genes,2 rRNA genes and the D-loop.The length of each coding gene sequence for every sample was relatively consistent,and the difference in length of the mitochondrial genome was mainly caused by the difference in D-loop length(975-1314 bp).Based on the 13 PCG and 2 rRNA gene sequences,phylogenetic analysis combined the sequences in present study with published closely related mitogenomes showed that the goby was divided into 3 major clades.Samples from Taihu Lake,Chaohu Lake and Nansi Lake were clustered into one clade while samples from the Nandu River Estuary or the Pearl River Estuary were divided into two clades.Cladistic analysis depicted that T.cirratus was showed to form a paraphyletic group with Odontamblyopus rubicundus and Taenioides anguillaris.The uplift of the Qinghai-Tibet Plateau and the formation of the Asian monsoon that brought about changes in water system pattern in China were the likely causes for the formation of T.cirratus current phylogenetic status.The finding provides crucially important basis for the resource conservation and further taxonomic clarification of T.cirratus.

关 键 词：须鳗虾虎鱼 线粒体基因组 系统发育 

分 类 号：S917.4[农业科学—水产科学] Q959.483[生物学—动物学]