基于蛋白指纹图谱的阳澄湖大闸蟹真伪鉴别研究  

Authentication of Suzhou Yangcheng Lake Hairy Crab Based on Protein Fingerprint

作　　者：张雅涵 张有勇 袁丹 徐敏 曹叶中 ZHANG Yahan;ZHANG Youyong;YUAN Dan;XU Min;CAO Yezhong(Suzhou Intellectual Property Protection Center,Jiangsu Suzhou 215100,China;Suzhou Institute for Food Control,Jiangsu Suzhou 215100,China)

机构地区：[1]苏州市知识产权保护中心,江苏苏州215100 [2]苏州市食品检验检测中心,江苏苏州215100

出　　处：《中国农业科技导报》2023年第11期80-89,共10页Journal of Agricultural Science and Technology

基　　金：苏州市科技项目(SNG201928)。

摘　　要：蟹螯肌肉组织蛋白质种类丰富,以不同强度的质谱峰出现在质谱图中,提取蟹鳌肌肉组织蛋白,利用基质辅助激光解析电离飞行时间质谱(matrix-assisted laser desorption/ionization time-of-flight mass spectrometry,MALDI-TOF MS)技术,对蟹鳌肌肉组织蛋白质谱进行特征指标分析,找到特征指标,可实现蟹鳌肌肉组织蛋白定性定量检测。通过分析蟹鳌组织蛋白的MALDI-TOF MS图谱规律,解析不同品类螃蟹蛋白质分子指纹图谱,并配合机器深度学习算法对数据库进行深度分析,建立阳澄湖大闸蟹的同源性分析方法。结果表明,不同生长环境的大闸蟹蟹鳌组织蛋白质谱图有不同的表达。m/z(质荷比,mass-to-charge ratio)范围在10000内均有明显差异,扫描结果显示,低质量区(m/z<10000)出峰强度较高且分辨率较高,该区域可以作为区分阳澄湖大闸蟹与其他类别湖蟹的质谱分辨区。以上结果表明,可以通过MALDI-TOF MS技术对不同产地大闸蟹的差异性蛋白质的表达进行分析,从而鉴别大闸蟹的产地。Crab chelate muscle tissue protein is rich in species and appears in the mass spectrum with different intensity peaks.Crab chelate muscle tissue protein is extracted,and the characteristic index of crab chelate muscle tissue protein spectrum was analyzed by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry(MALDI-TOF MS)technology,and the characteristic index is found to achieve the qualitative and quantitative detection of crab chelate muscle tissue protein.In this study,protein molecular fingerprint databases of different categories of crabs were established by analyzing the histoprotein MALDI-TOF MS technology,and the database was deeply analyzed with machine deep learning algorithm to establish the homology analysis method of Yangcheng Lake hairy crabs.The results showed that the protein profiles of hairy crabs in different growing environments showed different expressions.There was an obvious difference when m/z(mass-charge ratio,m/z)was less than 10000,the scanning results showed that the low m/z region(<10000)had higher peak intensity and higher submarking rate,this region could be used as the mass spectrum resolution region to distinguish Yangcheng Lake hairy crabs from other lake crabs.Above results showed MALDI-TOF MS technique could be used to analyze the different protein expressions of hairy crabs from different places,which could play a role in identifying hairy crab from different places.

关 键 词：MALDI-TOF质谱定量技术 阳澄湖大闸蟹 同源性分析 

分 类 号：S963[农业科学—水产养殖]