黄芩提取物外敷对糖尿病性溃疡大鼠创面影响的研究  

Effects of External Application of Scutellaria Baicalensis Extract on Wounds Surface of Diabetes Ulcer Rats

作　　者：于天一[1,2] 梁学威 YU Tianyi;LIANG Xuewei(Heilongjiang University of Chinese Medicine,Harbin 150040,China;The Third Affiliated Hospital of Heilongjiang University of Chinese Medicine,Harbin 150030,China;The First Affiliated Hospital of Heilongjiang University of Chinese Medicine,Harbin 150040,China)

机构地区：[1]黑龙江中医药大学,黑龙江哈尔滨150040 [2]黑龙江中医药大学附属第三医院,黑龙江哈尔滨150030 [3]黑龙江中医药大学附属第一医院,黑龙江哈尔滨150040

出　　处：《中医药学报》2023年第11期43-46,共4页Acta Chinese Medicine and Pharmacology

基　　金：黑龙江省博士后基金项目(LBH-Z17178);黑龙江省中医药科研项目(ZHY2022-159,ZHY2023-118)。

摘　　要：目的:观察黄芩提取物外敷对大鼠糖尿病性溃疡的治疗作用,并尝试从抗炎、抗氧化方面分析其作用机制。方法:取Wistar大鼠30只,随机分为实验组、模型组和空白组,每组10只,其中实验组、模型组建立糖尿病性溃疡模型,创面置于大鼠背部,空白组仅在相应部位进行备皮不建模。实验组采用黄芩提取物外敷创面局部,每2 d换药1次,换药后使用细绳限制行动3 h,防止其抓咬床面敷料,3 h过后自由行动。于治疗第30天观察各组创面面积、临床炎症指标(hs-CRP)、创面局部促炎因子(TNF-α、IL-6)、创面局部抑炎因子(IL-4、IL-10)、抗氧化指标(SOD、GSH)水平。结果:与模型组比较,实验组创面面积缩小、临床炎症指标降低、创面组织促炎因子水平下降的同时抑炎因子水平升高、创面组织抗氧化指标水平升高,差异均具有统计学意义(P<0.05)。结论:黄芩苷外敷治疗糖尿病性溃疡具有较好的治疗作用,主要体现在缩小创面面积和降低临床炎症指标方面,该作用可能与其抗氧化和炎症抑制作用有关。Objective:To observe the therapeutic effect of scutellaria baicalensis extract on diabetic ulcer in rats,and to analyze its mechanism from anti-inflammatory and antioxidant aspects.Methods:Thirty SD rats were randomly divided into an experimental group,a control group and a blank group,with 10 rats in each group.The diabetic ulcer model was established in the experimental group and the control group,and the wound was placed on the back of the rat.In the blank group,skin preparation was performed only in the corresponding parts without modeling.In the experimental group,the scutellaria baicalensis extract was applied externally to the wound surface,and the dressing was changed every 2 days.Thin ropes were used to restrict the movement of rats for 3 hours after each dressing change.On the 30th day after the treatment,the wound area,clinical inflammatory index(hs-CRP),local pro-inflammatory factors(TNF-αand IL-6),local anti-inflammatory factors(IL-4 and IL-10),and antioxidant index(SOD,GSH)levels in each group were observed.Results:Compared with the control group,the wound area,the clinical inflammatory indexes,and the levels of pro-inflammatory factors in the wound tissue in the experimental group decreased,while the levels of anti-inflammatory factors,and the levels of antioxidant indexes in the wound tissue increased.The differences were statistically significant(P<0.05).Conclusion:The external application of baicalin in the treatment of diabetic ulcer shows a good therapeutic effect,which is mainly reflected in reducing the wound area and downgrading the clinical inflammatory indexes.This effect may be related to its antioxidant and inflammatory inhibitory actions.

关 键 词：黄芩提取物 黄芩苷 糖尿病性溃疡 

分 类 号：R285.5[医药卫生—中药学]