水流速度对饥饿期中华绒螯蟹亲蟹能量代谢的影响  

Effects of different watervelocities on energy metabolism of parent Eriocheir sinensis under starvation

作　　者：李春波 沈晨晨 冯广朋[1,2] 赵峰[1] 张涛[1] 杨刚[1] LI Chun-bo;SHEN Chen-chen;FENG Guang-peng;ZHAO Feng;ZHANG Tao;YANG Gang(East China Sea Fisheries Research Institute,Chinese Academy of Fishery Sciences/Shanghai Engineering Research Center of Fisheries Resources Enhancement and Ecological Restoration of the Yangtze Estuary,Shanghai 200090,China;College of Fisheries and Life Science,Shanghai Ocean University,Shanghai 201306,China)

机构地区：[1]中国水产科学研究院东海水产研究所,上海长江口渔业资源增殖和生态修复工程技术研究中心,上海200090 [2]上海海洋大学水产与生命学院,上海201306

出　　处：《淡水渔业》2023年第6期52-57,共6页Freshwater Fisheries

基　　金：国家重点研发计划课题(2020YFD0900805);国家自然科学基金面上项目(31972795);上海市中华绒螯蟹现代农业产业技术体系项目(沪农科产字2017-4号)。

摘　　要：为掌握中华绒螯蟹(Eriocheir sinensis)亲蟹放流时的生态需求特征,利用Loligo systems装置测定饥饿期亲蟹的耗氧率和饥饿状态下最大耐流速度,并对不同水流速度处理1 h后测定亲蟹的肌肉、肝胰腺和鳃组织的琥珀酸脱氢酶(SDH)、丙酮酸激酶(PK)和己糖激酶(HK)活性。结果显示:在饥饿4 d后,亲蟹耗氧率趋于稳定,不同水流速度下亲蟹耗氧率均具有显著性差异,且亲蟹最大耐流速度为40 cm/s。肌肉和鳃中SDH活性在20 cm/s和30 cm/s水流速度下明显高于静止组;肌肉中PK和HK活性以及肝胰腺中PK活性在各流速组明显高于静水组。因此,在亲蟹人工放流时可选择水流速度较慢且食物充足的水域进行放流,减少自身能量的消耗,有效提高存活率。The oxygen consumption rate and the maximum water resistance velocity of parent crabs were measured by Loligo systems to obtain the optimal water velocity at the releasing sites of parent crabs.Meanwhile,the activity of succinate dehydrogenase(SDH),pyruvate kinase(PK)and hexokinase(HK)in muscle,hepatopancreas and gills were measured.These results suggested that the oxygen consumption rate of parent crabs tended to be stable after four days of starvation,and the maximum water resistance velocity of parent crabs was 40 cm/s.The activity of SDH in muscle and gill was significantly higher in the 20cm/s and 30 cm/s groups than that in the hydrostatic group.The activity of PK and HK in the muscle and the activity of PK in the hepatopancreas were significantly higher in each water velocity group than in the hydrostatic group.Therefore,when the parent crabs were released artificially,the water area with slow water velocity and sufficient food should be selected for release to reduce the energy consumption and effectively improve the survival rate.

关 键 词：中华绒螯蟹(Eriocheir sinensis) 水流 饥饿 耗氧率 能量代谢 

分 类 号：S937[农业科学—渔业资源]