Cu、Cd单一胁迫下双齿围沙蚕基因表达差异研究  

Transcriptomic analysis of gene expression of Perinereis Aibuhitensis during single Cu,Cd stress

作　　者：李聃 张来军 LI Dan;ZHANG Laijun(College of Ecology and Environment,Hainan Tropical Ocean University,Sanya 572022,China;School of Fisheries and Life Science,Hainan Tropical Ocean University/Hainan Key Laboratory of Herpetological Research,Sanya 572022,China)

机构地区：[1]海南热带海洋学院生态环境学院,海南三亚572022 [2]海南热带海洋学院水产与生命学院/海南省两栖爬行动物研究重点实验室,海南三亚572022

出　　处：《海洋环境科学》2023年第5期748-757,共10页Marine Environmental Science

基　　金：海南省自然科学基金项目(313050)。

摘　　要：为探讨双齿围沙蚕(Perinereis aibuhitensis)响应重金属胁迫的作用机制,本文以Cu、Cd为胁迫因子,利用转录组测序技术研究在不同浓度的Cu(0.01 mg/L、0.5 mg/L和1 mg/L)、Cd(0.005 mg/L、0.25 mg/L、0.5 mg/L)胁迫下双齿围沙蚕的差异表达基因。分析结果显示,6个处理组共有的差异表达基因有101条,Cu处理组共有99个差异表达基因,Cd处理组共有42个差异表达基因;Cu、Cd处理组差异表达基因均在谷胱甘肽代谢、过氧化物酶体、细胞色素P450对外源物质代谢、ABC转运蛋白等通路显著富集(P<0.05),表明这几条通路在双齿围沙蚕响应Cu、Cd胁迫的抗逆过程中起到重要作用。同时,本研究筛选出CYP4f4、CYP1A4、Cyp3a25、CYP2B4、cyp17a1、Cyp4f14等响应重金属Cu、Cd胁迫的抗性基因。本研究结果揭示了Cu、Cd胁迫对双齿围沙蚕在基因层面上的影响和差异,为深入研究双齿围沙蚕响应重金属胁迫的分子机制以及抗性基因筛选和克隆提供数据支持和理论参考。In order to explore the mechanism of response to heavy metal stress of Perinereis aibuhitensis,copper and cadmium were used as stress factors,and transcriptome sequencing technology was used to study the gene expression differences of Perinereis aibuhitensis under the stress of different concentrations of copper(0.01 mg/L,0.5 mg/L,1 mg/L)and cadmium(0.005 mg/L,0.25 mg/L,0.5 mg/L).The analysis showed that there were 101 differently expressed genes in the six treatment groups,99 differently expressed genes in the Cu treatment group and 42 differently expressed genes in the Cd treatment group;the differentially expressed genes in the Cu and Cd treatment groups were significantly enriched(P<0.05)in the glutathione metabolism,peroxisome,Metabolism of xenobiotics by cytochrome P450,and ABC transporter protein pathways.These pathways play an important role in the response to Cu and Cd stresses in the Perinereis aibuhitensis.CYP4f4,CYP1A4,Cyp3a25,CYP2B4,cyp17a1 and Cyp4f14 were screened for resistance genes in response to heavy metal Cu and Cd stresses in this study.The results of this study reveal the effects and differences of Cu and Cd stress on Perinereis aibuhitensis at the genetic level,and provide data support and theoretical reference for the in-depth study of the molecular mechanisms of Perinereis aibuhitensis in response to heavy metal stress and the screening and cloning of resistance genes.

关 键 词：CU CD 双齿围沙蚕 转录组测序 

分 类 号：Q178.53[生物学—水生生物学] X171.5[生物学—普通生物学]