黄海北部庄河沿岸米草属植物种类鉴定及防治建议  

Molecular identification and control suggestions of Spartina in Zhuanghe coastal zone of the Northern Yellow Sea

作　　者：于彩芬[1] 陈英旭 陈鹏飞[1] 张帆[1] 姜洋[2] 刘长安[1] YU Caifen;CHEN Yingxu;CHEN Pengfei;ZHANG Fan;JIANG Yang;LIU Chang’an(National Marine Environmental Monitoring Center,Dalian 116023,China;Dalian Ocean University,Dalian 116023,China)

机构地区：[1]国家海洋环境监测中心,辽宁大连116023 [2]大连海洋大学,辽宁大连116023

出　　处：《海洋环境科学》2023年第5期774-779,共6页Marine Environmental Science

基　　金：科技基础资源调查专项项目(2022FY100300)。

摘　　要：米草属植物是目前我国沿海地区最严重的外来入侵植物,已经严重威胁海岸带的生物多样性和生态系统的稳定性。为明确我国黄海北部庄河沿岸米草属植物的种类,提出有针对性的防治措施,本研究利用分子生物学技术对其进行物种鉴定。以植物新鲜叶片的总DNA为模板,采用引物S65-F(5’-ACACTACCCTGATCATCCTCT-3’)与S65-R(5’-TCTGGCTGGATTGTTGTCTGT-3’)进行PCR扩增,采用通用引物ITS1(TCCGTAGGTGAACCTGCGG)和ITS4(TCCTCCGCTTATTGATATGC)同时进行扩增进而验证鉴定结果,确定黄海北部庄河沿岸米草属植物种类为大米草(Spartina anglica)。鉴于大米草入侵会对沿海生物多样性和生态系统造成诸多影响,建议地方政府强化对大米草的防治工作,采取积极有效的措施对大米草进行综合治理。Spartina is the most invasive alien plant along the coast of China,which has seriously threatened the biodiversity and ecosystem stability in coastal zone.In order to identify the species of Spartina in Zhuanghe coastal zone of the Northern Yellow Sea,and put forward targeted control measures,in this paper,the species was identified using molecular detection technology.PCR amplification was performed from total DNA of fresh plant leaves using primers S65-F/S65-R and universal primers ITS.Among them,S65 forward rimer sequence is 5’-ACACTACCCTGATCATCCTCT-3’and S65 reverse primer sequence is 5’-TCTGGCTGGATTGTTGTCTGT-3’.The universal primers are ITS1(TCCGTAGGTGAACCTGCGG)and ITS4(TCCTCCGCTTATTGATATGA).The results showed that Spartina was identified as Spartina anglica in Zhuanghe coastal zone of the Northern Yellow Sea.It is suggested that local governments should strengthen the prevention and control of Spartina anglica and carry out comprehensive management in view of the impacts of Spartina anglica invasion on biodiversity and coastal ecosystems.

关 键 词：米草属植物 分子鉴定 生物入侵 防治 黄海北部 

分 类 号：S451[农业科学—植物保护]