激素和逆境胁迫对大豆维生素E和γ-TMT表达的影响  

Effects of Hormonal and Adversely Stress on Vitamin E and γ-TMT Gene Expression in Soybeans

作　　者：白苗 田雯青 武帅 王敏[1] 王利祥 岳爱琴[1] 牛景萍 张永坡 高春艳 张武霞 郭数进[1] 杜维俊[1] 赵晋忠 BAI Miao;TIAN Wen-qing;WU Shuai;WANG Min;WANG Li-xiang;YUE Ai-qin;NIU Jing-ping;ZHANG Yong-po;GAO Chun-yan;ZHANG Wu-xia;GUO Shu-jin;DU Wei-jun;ZHAO Jin-zhong(College of Agronomy,Shanxi Agricultural University,Taigu 030801;Department of Basic Science,Shanxi Agricultural University,Taigu 030801;College of Life Science,Shanxi Agricultural University,Taigu 030801)

机构地区：[1]山西农业大学农学院,太谷030801 [2]山西农业大学基础部,太谷030801 [3]山西农业大学生命科学学院,太谷030801

出　　处：《生物技术通报》2023年第10期148-162,共15页Biotechnology Bulletin

基　　金：中央引导地方科技发展资金项目(YDZJSX2022A035);国家重点研发计划(2021YFD1600601-2);山西省种业创新联合攻关项目(2023xczx03);山西农业大学育种工程项目(YZGC096);山西省自然基金项目(201901D111225)。

摘　　要：明确大豆维生素组分含量及合成相关基因γ-TMT对激素和逆境胁迫的响应,为维生素E参与逆境胁迫研究开辟新思路。以山西省主栽品种晋大88为试验材料,在激素、逆境胁迫条件下对不同组织的维生素E组分含量、生理指标、γ-TMT表达模式进行分析。结果表明,维生素E主要存在于叶中,茎中有少量,根中不存在。在激素及盐等胁迫条件下,叶和茎的总维生素E含量小于对照,但γ-生育酚含量在Na Cl、H_(2)O_(2)、ABA胁迫下含量高于对照。在激素和逆境胁迫环境下,大豆植株的生长被显著抑制,株高、鲜重、干重、SPAD值以及含水量皆小于对照。基因组数据挖掘到3个γ-TMT,启动子中均存在许多胁迫应答和激素响应元件,在激素和逆境胁迫下,γ-TMT1-γ-TMT3在根、叶中的表达均发生上调或下调。在NaCl胁迫条件下,大豆叶中γ-生育酚含量增加,而γ-TMT2、γ-TMT3的表达明显下调,推测γ-TMT2和γ-TMT3可能影响盐胁迫条件下维生素组分含量。激素和逆境胁迫显著抑制大豆茎和叶中维生素E的含量,进而抑制植株的生长,影响了大豆γ-TMT表达和维生素E组分。The response of vitamin content and synthesis-related gene γ-TMT to hormonal and stress stress was clarified,which opened up new ideas for vitamin E to participate in the study of adversity stress.Taking Jinda 88,the main cultivars of Shanxi province,as the experimental materials,the vitamin E component content,physiological indexes and γ-TMT expression patterns in different tissues were analyzed under hormonal and adversity stress.The results showed that vitamin E was mainly present in the leaves,a small amount in stems,and absent in roots,and the total vitamin E content in leaves and stems was smaller than that in control under stress conditions such as hormones and salts,but the content of γ-tocopherols under Na Cl,H_(2)O_(2) and ABA stress was higher than that of the control.Under hormonal and adversity stress,the growth of soybean plants was significantly inhibited,and the plant height,fresh weight,dry weight,SPAD value and water content were all lower than those of the control.Genomic data were mined to 3 γ-TMTs,and there were many stresses response and hormone response elements in the promoters,and the expression of γ-TMT1-γ-TMT3 in roots and leaves was upregulated or down-regulated under hormonal and adversity stress.Under Na Cl stress,the content of γ-tocopherol in soybean leaves increased,while the expression of γ-TMT2 and γ-TMT3 was significantly down-regulated,and it was speculated that γ-TMT2 and γ-TMT3 may affect the content of vitamin components under salt stress.Hormonal and adverse stress significantly inhibited the contents of vitamin E in soybean stems and leaves,which in turn inhibited plant growth,i.e.,affected soybean γ-TMT expression and vitamin E components.

关 键 词：大豆 维生素E γ-TMT 激素 逆境 胁迫 

分 类 号：S565.1[农业科学—作物学]