一株林麝源弗格森埃希菌的分离鉴定及耐药性  

Identification and Drug Resistance Analysis of Escherichia fergusonii from Forest Musk Deer

作　　者：彭茜翎 王如意[1] 乔美萍 周磊[4] 王洪永 蒋本模 吴杰[4] 杨杰[4] 其美拉姆 颜其贵[1,2] PENG Qianling;WANG Ruyi;QIAO Meiping;ZHOU Lei;WANG Hongyong;JIANG Benmo;WU Jie;YANG Jie;Qimei Lamu;YAN Qigui(College of Veterinary Medicine,Sichuan Agricultural University,Chengdu,611130,China;Key Laboratory of Animal Disease and Human Health of Sichuan Province,College of Veterinary Medicine,Sichuan Agricultural University,Chengdu,611130,China;Qingchuan Jiuyao Musk Deer Industry Development,Guangyuan,628100,China;Sichuan Institute of Musk Deer Breeding,Dujiangyan,611845,China;Fengxian Fengchun Jiminkexin Technology Breeding,Baoji,721000,China;Shangchayu Town Agriculture and Animal Husbandry Comprehensive Service Center,Nyingchi,860614,China)

机构地区：[1]四川农业大学动物医学院,成都611130 [2]四川农业大学动物医学院动物疫病与人类健康四川省重点实验室,成都611130 [3]青川九药麝业开发有限公司,广元628100 [4]四川养麝研究所,都江堰611845 [5]凤县逢春济民可信科技养殖有限公司,宝鸡721000 [6]上察隅镇农牧综合服务中心,林芝860614

出　　处：《野生动物学报》2023年第4期870-877,共8页CHINESE JOURNAL OF WILDLIFE

基　　金：陕西省重点研发计划项目(2020ZDLSF05-01);四川省科技计划项目(2021JDZH0024)。

摘　　要：为探究林麝(Moschus berezovskii)的死亡原因,无菌采集死亡林麝脏器样本,通过细菌分离纯化、生化鉴定、16S rDNA基因测序、纸片扩散法和耐药基因检测方法对细菌进行分离、鉴定及分析分离菌株的耐药性。结果表明:从死亡林麝脏器样本中分离得到的菌株为弗格森埃希菌(Escherichia fergusonii),该分离菌株对头孢噻肟、头孢噻吩和青霉素耐药,对氨基糖苷类、喹诺酮类及氨基青霉素类药物敏感;携带耐药基因bla-OXA_(1/4/30)、bla-CTX-M和bla-SHV。研究结果可为林麝养殖过程中致病性弗格森埃希菌的预防和治疗提供参考。To investigate the death cause of a forest musk deer(Moschus berezovskii),samples of the dead forest musk deer were collected aseptically for bacterial isolation and drug resistance analysis.The isolate was identified by bacterial isolation and purification,biochemical identification,16S rDNA gene sequencing,disc diffusion test and antibiotic resistance genes detection.The results showed that Escherichia fergusonii was isolated from the samples of the dead deer.Drug sensitive test confirmed that the isolate was resistant to cefotaxime,cephalothin and penicillin,while it was sensitive to aminoglycosides,quinolones and aminopenicillins.The analysis of resistance genes detection showed that bla-OXA1/4/30,blaCTX-M and bla-SHV were all detected.In conclusion,this study provides a reference to prevention and treatment of pathogenic E.fergusonii in the cultivation of forest musk deer.

关 键 词：林麝 弗格森埃希菌 ESBLS Β-内酰胺类抗生素 

分 类 号：S858.9[农业科学—临床兽医学]