基于桑格测序法建立高鼻羚羊鉴定方法的可行性研究  

Feasibility Study for the Establishment of a Saiga Antelope Identification Method Based on Sanger Sequencing

作　　者：沙才华 廖秀云 陈轩 周傲白雪 蒋晓霞 徐博文 黄海超 赵福振 邵建宏 SHA Caihua;LIAO Xiuyun;CHEN Xuan;ZHOU Aobaixue;JIANG Xiaoxia;XU Bowen;HUANG Haichao;ZHAO Fuzhen;SHAO Jianhong(Technical Center of Gongbei Customs District P.R.China,Key Laboratory of Exotic Disease Detection for Nation,Key Laboratory of Foot and Mouth Disease and Swine Fever Detection for Nation,Zhuhai,519001,China)

机构地区：[1]拱北海关技术中心,国家外来病检测重点实验室,国家口蹄疫猪瘟检测重点实验室,珠海519001

出　　处：《野生动物学报》2023年第4期890-895,共6页CHINESE JOURNAL OF WILDLIFE

摘　　要：为有效鉴定高鼻羚羊(Saiga tatarica)源性成分,基于细胞色素c氧化酶亚型Ⅰ基因设计特异性引物对高鼻羚羊角、高鼻羚羊角粉,以及与其种属相近或形态学相似的动物角和动物组织的DNA进行PCR扩增,对扩增产物进行桑格测序和序列分析,并对高鼻羚羊的物种分类问题进行探讨。结果表明:所有对照样品均未见扩增条带,阳性样品与NCBI基因数据库参考序列的相似性均大于99.42%,说明桑格测序法能准确鉴定高鼻羚羊成分,但由于证据不足,无法明确该方法能否进一步区分高鼻羚羊与蒙古高鼻羚羊(S.t.mongolica)。In order to effectively identify the presence of saiga antelope(Saiga tatarica)-derived ingredient,this study designed specific primers based on the cytochrome c oxidase subunit Ⅰ gene and then used them to amplify DNA from saiga antelope horns and saiga antelope horn powder,as well as from animal horns and tissues that are comparable in species or morphology.The amplified products were sequenced and analyzed,and the species classification of saiga antelope was discussed.Results showed that no amplification bands were observed in the control samples,and the similarity between the positive samples and the reference sequence in NCBI GenBank was greater than 99.42%.This method can accurately identify the presence of saiga antelope components,but due to lack of evidence,it is unclear whether it can further distinguish between saiga antelope and Mongolian saiga antelope(S.t.mongolica).

关 键 词：高鼻羚羊 细胞色素c氧化酶亚型Ⅰ 测序 鉴定 

分 类 号：Q523.8[生物学—生物化学]