尿素[^(14)C]胶囊中^(14)C核素的鉴别  

Identification of ^(14)C Nuclides in ^(14)C-urea Capsules

作　　者：马彦 张辉 杨永刚 马莉娜[1,2] 王亚东 宋丽娟[1,2] 王路生 戴雄新 MA Yan;ZHANG Hui;YANG Yonggang;MA Lina;WANG Yadong;SONG Lijuan;WANG Lusheng;DAI Xiongxin(China Institute for Radiation Protection,Taiyuan 030006,China;Shanxi Provincial Key Laboratory for Translational Nuclear Medicine and Precision Protection,Taiyuan 030006,China)

机构地区：[1]中国辐射防护研究院,山西太原030006 [2]核药研发转化与精准防护山西省重点实验室,山西太原030006

出　　处：《原子能科学技术》2023年第11期2162-2169,共8页Atomic Energy Science and Technology

基　　金：中国辐射防护研究院青年基金(YQ22000725)。

摘　　要：^(14)C-尿素呼气试验是幽门螺杆菌检测的“金标准”,检测时受试者需要服用^(14)C标记的尿素胶囊,为保证药品质量,需对尿素[^(14)C]胶囊中的有效成分^(14)C进行核素鉴别,目前尚缺乏简单可行的核素鉴别方法。本工作建立了一种基于液闪测量的尿素[^(14)C]胶囊中^(14)C核素鉴别方法。通过研究氧气和乙醇对液闪β谱图的影响,确定了低淬灭^(14)C样品制备方法,去除氧气时间大于10 min,乙醇添加量应小于1.67%,样品“有效期”为30 min。在相同测量条件下,通过比较低淬灭样品和无淬灭^(14)C标准源的β谱图的特征点,实现了尿素[^(14)C]胶囊中^(14)C核素的鉴别。采用所建立的方法对上海某公司3批次尿素[^(14)C]胶囊中的^(14)C进行鉴别,证明了建立的^(14)C核素鉴别方法可行有效。Helicobacter pylori is a type of bacteria that is commonly found in the stomach lining and is known to cause gastritis,gastric ulcers,and gastric cancer.Detection and diagnosis of Helicobacter pylori infection are crucial for effectively eradicating the pathogen.Currently,the ^(14)C-urea breath test is widely considered the gold standard for detecting the infection.During the test,patients ingest capsules containing ^(14)C-labeled urea.It is important to identify the active ingredient,^(14)C,in the ^(14)C-urea capsules to ensure the quality and safety of the medication.Unfortunately,there is no clear method provided by the Chinese Pharmacopoeia or the United States Pharmacopoeia for identifying the ^(14)C nuclide.To address this gap,a method for identifying the ^(14)C nuclide in ^(14)C-urea capsules using liquid scintillation counter measurement was developed in this paper.In the experiment,a solution of ^(14)C-urea was mixed with ethanol and low-quenching scintillation cocktails,and then argon gas was used to remove oxygen and prepare a low-quenching sample.The liquid scintillation counter was used to measured theβspectrum,and five characteristic points were selected for comparison with theβspectrum of a standard non-quenched ^(14)C source.The identified characteristic points included the maximum apparent energy value,the apparent energy value at the right end of the half-peak height,the apparent energy value at the right end of the 90%peak height,the 90%peak energy width,and half-height width.Successful identification of the ^(14)C nuclides is achieved by comparing theβspectrum of the low-quenching sample with the standard non-quenched ^(14)C source.Oxygen and ethanol are the main sources of quenching during the preparation of low-quenching samples.However,the experimental study shows that the dissolved oxygen in the scintillation cocktails could be effectively removed by flowing argon gas for 10 minutes,which significantly reduces the quenching degree and extends the validity period of the sample to 30 minu

关 键 词：核素鉴别 液闪 Β谱 ^(14)C 

分 类 号：TL92[核科学技术—核燃料循环与材料] O608.1[理学—化学]