基于诱变和基因组重排选育木质素酶高产菌株的研究  

Study on the breeding of high-yield lignin enzyme strains based on mutagenesis and genome rearrangement

作　　者：鲁云风[1] 王丰园 LU Yun-feng;WANG Feng-yuan

机构地区：[1]南阳师范学院生命科学与农业工程学院,河南南阳473061 [2]三门峡市产业技术研究院,河南三门峡472099

出　　处：《饲料研究》2023年第21期76-83,共8页Feed Research

基　　金：河南省高等学校重点科研项目(项目编号:20A180022);国家重点实验室开放课题(项目编号:KFKT2018002)。

摘　　要：试验旨在高效降解木质纤维素,采用诱变和基因组重排选育木质素酶高产菌株。以黄孢原毛平革菌(Phanerochaete chrysosporium)为原始菌株,采用常温常压等离子体(ARTP)和硫酸二乙酯(DES)进行复合诱变,以苯胺蓝脱色圈法和愈创木酚显色圈法相结合快速筛选黄孢原毛平革菌突变菌株,作为基因组重排的亲本菌株,通过两轮的基因组重排,并经过遗传稳定性检测。结果显示,试验筛选得到1株木质素酶高产菌株PR2-24,其木质素过氧化物酶活、锰过氧化物酶活、漆酶酶活分别为11.34、88.35、10.82 U/mL,分别较原始菌株提高了181%、171%、203%。研究表明,试验筛选的木质素酶高产菌株为微生态饲料添加剂的研发提供了参考。The study was to efficiently degrade lignocellulose,ligninase high-producing strains were screened and bred by mutation and genome rearrangement.Phanerochaete chrysosporium was used as an original strain and treated with atmospheric room temperature plasma(ARTP)and diethyl sulphate(DES).Mutant strains with higher enzyme productivity were screened by the combination of aniline blue decolorization circle method and guaiacol color circle method and used as starting strains for genome shuffling.Through two rounds of genome rearrangement and genetic stability test.The results showed that the high-yield lignin enzyme fusion PR2-24 was obtained,and its lignin peroxidase activity,manganese peroxidase activity and laccase activity were 11.34,88.35 and 10.82 U/mL respectively,which were 181%,171%and 203%higher than those of the original strain.The results showed that the high-yield lignin enzyme strains screened by the test provided a reference for the research and development of microecological feed additives.

关 键 词：黄孢原毛平革菌 诱变 基因组重排 木质素酶 

分 类 号：S816[农业科学—饲料科学]