上调miR-590-3p对胰腺癌细胞侵袭转移的作用及影响机制  

Effect of Up Regulating miR-590-3p on Invasion and Metastasis of Pancreatic Cancer Cells and Its Mechanism

作　　者：王亚东 王俊峰[3] WANG Ya-dong;WANG Jun-feng(General Surgery Department of Wuhu Traditional Chinese Medicine Hospital,Wuhu Anhui 241000,China;Anhui Medical University,Hefei Anhui 230032,China;General Surgery Department of Yijishan Hospital,Wannan Medical College,Wuhu Anhui 241001,China)

机构地区：[1]芜湖市中医医院普外科,安徽芜湖241000 [2]安徽医科大学,安徽合肥230032 [3]皖南医学院弋矶山医院普外科,安徽芜湖241001

出　　处：《中华养生保健》2023年第23期4-8,共5页CHINESE HEALTH CARE

基　　金：芜湖市卫健委项目(WHWJ2021y052)。

摘　　要：目的观察miR-590-3p基因表达对人胰腺癌Capan-2细胞侵袭转移能力的影响。方法人胰腺癌Capan-2细胞分为两组:阴性对照组(NC组)及转染miR-590-3p mimics组(miR-590-3p mimics组)。采用qRT-PCR法检测正常胰腺导管上皮细胞hTERT-HPNE以及胰腺癌Capan-2细胞中miR-590-3p的表达,通过流式细胞术检测miR-590-3p对Capan-2细胞凋亡的影响,通过MTT实验检测miR-590-3p对Capan-2细胞增殖的影响,通过划痕实验检测细胞迁移能力的变化,通过Transwell实验检测细胞侵袭能力的变化,采用Western blot法检测AKT/mTOR通路相关蛋白表达变化。结果胰腺癌Capan-2细胞较正常胰腺导管上皮细胞中miR-590-3p表达水平显著降低,差异有统计学意义(P<0.05);与NC组相比,miR-590-3p mimics组miR-590-3p mRNA表达水平显著升高,差异有统计学意义(P<0.05);流式细胞术实验结果显示,与NC组相比,miR-590-3p mimics组细胞凋亡水平显著升高,差异有统计学意义(P<0.05);细胞划痕及侵袭实验结果显示,与NC组相比,miR-590-3p mimics组细胞迁移和侵袭能力显著降低,差异有统计学意义(P<0.05);Western blot结果显示,与NC组相比,miR-590-3p mimics组中p-AKT和p-mTOR蛋白水平明显降低,差异有统计学意义(P<0.05)。结论上调miR-590-3p表达可增加胰腺癌细胞凋亡能力,抑制胰腺癌细胞迁移和侵袭能力。Objective To observe the eff ect of miR-590-3p gene expression on the invasion and metastasis of human pancreatic cancer Capan-2 cells.Methods Human pancreatic cancer Capan-2 cells were divided into two groups:negative control group(NC group)and miR-590-3p up-regulated group(miR-590-3p mimics group).The expression of miR-590-3p in human normal pancreatic ductal epithelial cells hTERT-HPNE and pancreatic cancer Capan-2 cells was detected by qRT PCR.The eff ect of miR-590-3p on the apoptosis of Capan-2 cells was detected by flow cytometry,the eff ect of miR-590-3p on the proliferation of Capan-2 cells was detected by MTT test,the change of cell migration ability was detected by scratch test,and the change of cell invasion ability was detected by Transwell test,the expression of AKT/mTOR pathway related proteins was detected by Western blot.Results The expression level of miR-590-3p in Capan-2 cells of pancreatic cancer was significantly lower than that in normal pancreatic duct epithelial cells,and the expression level of miR-590-3p mRNA in miR-590-3p mimics group was significantly higher than that in NC group(P<0.05).The results of flow cytometry showed that the level of apoptosis in miR-590-3p mimics group was significantly higher than that in NC group(P<0.05).The results of cell scratch and invasion test showed that the migration and invasion ability of miR-590-3p mimics group was significantly lower than that of NC group(P<0.05).Western blot results showed that compared with the NC group,the levels of p-AKT and p-mTOR proteins in the miR-590-3p mimics group were significantly reduced.Conclusion Up regulation of miR-590-3p expression can increase the apoptosis of pancreatic cancer cells and inhibit the migration and invasion of pancreatic cancer cells.

关 键 词：胰腺癌 miR-590-3p 侵袭 转移 

分 类 号：R657.5[医药卫生—外科学]