基于ISSR标记的蜂糖李亲缘关系分析  

Relatedness Analysis of Prunus salicina'Fengtangli'Based on ISSR Markers

作　　者：麦鹏坤 郑乾明 赵雅楠 张敏[2] 马玉华[2] MAI Pengkun;ZHENG Qianming;ZHAO Ya'nan;ZHANG Min;MA Yuhua(Key laboratory of Plant Resource Conservation and Germplasm Innovation in Mountainous Region(Ministry of Education),Collaborative Innovation Center for Mountain Ecology&Agro-Bioengineering(CICMEAB),College of Life Sciences/Institute of Agro-bioengineering,Guizhou University,Guiyang 550025,China;Institute of Fruit Tree Science,Guizhou Academy of Agricultural Sciences,Guiyang 550006,China)

机构地区：[1]贵州大学生命科学学院/农业生物工程研究院,山地植物资源保护与保护种质创新教育部重点实验室,山地生态与农业生物工程协同创新中心,贵阳550025 [2]贵州省农业科学院果树科学研究所,贵阳550006

出　　处：《种子》2023年第9期30-35,67,共7页Seed

基　　金：贵州省科技计划项目“贵州蜂糖李优质高产关键技术集成与示范”(黔科合支撑[2022]重点018号);贵州省高层次创新型百层次人才(黔科合平台人才-GCC[2022]025-1)。

摘　　要：以贵州省5个地区52份蜂糖李种质为供试群体,通过筛选出的10条ISSR核心引物对52份蜂糖李样品进行亲缘关系分析及遗传多样性评价。结果表明,10条核心引物在52份蜂糖李样品中共扩增出位点76个,平均每条引物扩增7.6个,其中多态性位点有73个,平均多态性位点比率为96.05%。UBC842与UBC856扩增位点最多,达10个,UBC834扩增位点最少,仅6个。等位基因数(Na)在1.6250~2.0000之间,均值1.8889,有效等位基因数(Ne)在1.3244~1.6965之间,均值1.5093,Nei's基因多样性指数(H)在0.2214~0.3916之间,均值0.3004,Shannon's信息指数(I)在0.3365~0.5714之间,均值0.4523。综合结果表明,供试蜂糖李样品遗传多样性水平比较高,筛选出的ISSR引物可用于研究蜂糖李的遗传多样性,可为蜂糖李新种质的培育与资源的保存提供参考和技术支持。52 Prunus salicina germplasm from 5 regions in Guizhou Province were used as the test population.10 ISSR core primers selected for the 52 Prunus salicina samples were conducted by relatedness analysis and genetic evaluation.The results showed that the 10 core primers amplified a total of 76 loci in the 52 Prunus salicina samples,with an average of 7.6 loci per primer,of which 73 loci were polymorphic,with an average polymorphism rate of 96.05%.UBC842 and UBC856 amplified the most loci,with 10 loci,while UBC834 amplified the least loci,with only 6 loci.The number of alleles(Na)ranged from 1.6250 to 2.0000 with a mean value of 1.8889,the effective number of alleles(Ne)ranged from 1.3244 to 1.6965 with a mean value of 1.5093,the Nei's gene diversity index(H)ranged from 0.2214 to 0.3916 with a mean value of 0.3004,and the Shannon's information index(I)ranged from 0.3365 to 0.5714 with a mean value of 0.4523.The overall results indicated that the genetic diversity of the test samples was relatively high and the selected ISSR primers could be used to study the genetic diversity of Prunus salicina,and could provide reference and technical support for the breeding of new germplasm and conservation of resources.

关 键 词：蜂糖李 ISSR 遗传多样性 聚类分析 

分 类 号：S662.3[农业科学—果树学]