基于GBS测序和连锁分析的藜麦单株粒重QTL定位  

QTL Mapping for Single Plant Grain Weight in Quinoa Based on GBS Sequencing and Linkage Analysis

作　　者：吴文强 代越琪 陈天青[1] 隋建枢[1] 罗永露 王伟[1] 何庆才[1] WU Wenqiang;DAI Yueqi;CHEN Tianqing;SUI Jianshu;LUO Yonglu;WANG Wei;HE Qingcai(Institute of Upland Crops,Guizhou Academy of Agricultural Sciences,Guiyang 550006,China;Guizhou Jinnong Science-technology Co.Ltd,Guiyang 550006,China)

机构地区：[1]贵州省农业科学院旱粮研究所,贵阳550006 [2]贵州金农科技有限责任公司,贵阳550006

出　　处：《种子》2023年第9期45-48,120,共5页Seed

基　　金：贵州省农科院基金项目(黔农科院青年科技基金[2021]24号);贵州省科技计划项目(黔科合支撑[2019]2300号);贵州省科技计划项目([2018]4005-4)。

摘　　要：单株粒重是影响藜麦产量的关键因素之一。为了解藜麦单株粒重形成的遗传基础,鉴定控制藜麦单株粒重变异的遗传区段,本研究以单株粒重差异较大的两个亲本构建的120份双亲分离群体为材料,基于GBS测序和表型鉴定结果,利用完备区间作图法鉴定控制藜麦单株粒重变异的QTL。结果定位到7个控制藜麦单株粒重的QTL,分别位于第3条染色体的qSPW3-1、qSPW3-2,第4条染色体的qSPW4,第5条染色体的qSPW5,第7条染色体的qSPW7-1、qSPW7-2,第12条染色体的qSPW12;单个QTL可解释6.09%~10.17%的表型变异,QTL的变幅为10.54~401.86 kb。The single plant grain weight is one of the key factors affecting the quinoa yield.In order to understand the genetic basis for the formation of single plant grain weight in quinoa and identify the genetic segment that controlled the variation of single plant grain weight in quinoa,this study used 120 parents separated populations constructed from two parents with significant differences in single plant grain weight as materials.Based on GBS sequencing and phenotypic identification results,the QTLs that controlled the variation of quinoa yield were identified using a complete inter region mapping method.The results showed that a total of 7 QTLs controlling single plant grain weight of quinoa were identified,namely qSPW3-1 and qSPW3-2 located on the third chromosome,qSPW4 located on the fourth chromosome,qSPW5 located on the fifth chromosome,qSPW7-1 and qSPW7-2 located on the seventh chromosome,and qSPW12 located on chromosome 12.A single QTL could explain phenotypic variations ranging from 6.09%to 10.17%,with QTL sizes ranging from 10.54 kb to 401.86 kb.

关 键 词：藜麦 单株粒重 GBS QTL 

分 类 号：S519[农业科学—作物学]