不同产地“宁杞1号”枸杞果实的多糖合成代谢差异  

The Difference of Polysaccharide Synthesis and Metabolism of ‘Ningqi No. 1’ Lycium Barbarum Fruits from Different Producing Areas

作　　者：马瑞雪 寇婷婷 孙霞芝 范艳丽[1] Ma Ruixue;Kou Tingting;Sun Xiazhi;Fan Yanli(College of Food Science and Engineering,Ningxia University,Yinchuan 750021)

机构地区：[1]宁夏大学食品科学与工程学院,银川750021

出　　处：《中国食品学报》2023年第9期295-305,共11页Journal of Chinese Institute Of Food Science and Technology

基　　金：国家自然科学基金项目(32160534)。

摘　　要：基于同位素标记相对和绝对定量(isobaric tags for relative and absolute quantity,iTRAQ)技术,以采自宁夏中宁的“宁杞1号”枸杞果实为样品,以采自新疆精河、内蒙古乌拉特前旗、甘肃瓜州和青海德令哈的“宁杞1号”枸杞果实为对照,进行差异蛋白组学的生物信息学分析。结果表明,新疆/宁夏组、内蒙古/宁夏组、甘肃/宁夏组和青海/宁夏组分别有446,166,966个和1015个差异表达蛋白(DEPs),与枸杞多糖合成代谢相关的DEPs共160个。进一步筛选得到23个与枸杞多糖相关DEPs,包括蔗糖磷酸合成酶、β-葡萄糖苷酶和α-半乳糖苷酶等。推测采自不同产地的枸杞果实的多糖合成代谢差异通路主要包括蔗糖、纤维素、半乳聚糖、半纤维素和果胶合成代谢过程,且这些过程均可通过UDP-葡萄糖链接在一起。通过蛋白组学分析枸杞多糖合成途径上的相关蛋白,为深入研究不同产地同种枸杞多糖调控机制提供理论参考。Based on iTRAQ(isobaric tags for relative and absolute quantity,iTRAQ)technology,the bioinformatics analysis of differential proteomics were carried out with the fruits of‘Ningqi No.1’wolfberry collected from Zhongning,Ningxia as samples and the fruits of‘Ningqi No.1’wolfberry collected from Jinghe,Xinjiang,wulateqian banner,Inner Mongolia,Guazhou,Gansu and Delingha,Qinghai as controls.The results showed that there were 446,166,966 and 1015 differentially expressed proteins(DEPs)in Xinjiang/Ningxia group,Inner Mongolia/Ningxia group,Gansu/Ningxia group and Qinghai/Ningxia group respectively,and 160 DEPs related to Lycium barbarum polysaccharide synthesis and metabolism.Through further screening,23 DEPs related to Lycium barbarum polysaccharides were obtained,including sucrose phosphate synthase,β-Glucosidase andα-Galactosidase,etc.It was speculated that the different pathways of polysaccharide anabolism in Lycium barbarum fruits from different producing areas mainly included sucrose,cellulose,galactan,hemicellulose and pectin anabolism,and these processes can be linked together with UDP-glucose.This study analyzed the related proteins in the synthesis pathways of Lycium barbarum polysaccharides by proteomics,which provides a theoretical basis for further study on the regulation mechanism of the same Lycium barbarum polysaccharide from different producing areas.

关 键 词：枸杞 枸杞多糖(LBP) 差异表达蛋白(DEPs) 多糖合成代谢途径 

分 类 号：R284.1[医药卫生—中药学]