Bisulfite-free mapping of DNA cytosine modifications:challenges and perspectives  

作　　者：Yanfang Du Ying Tang Bingqian Lin Xiaochen Xue Yafen Wang Yibin Liu 

机构地区：[1]College of Chemistry and Molecular Sciences,Wuhan University,Wuhan 430072,China [2]School of Public Health,Wuhan University,Wuhan 430071,China [3]Taikang Center for Life and Medical Sciences,Wuhan University,Wuhan 430071,China

出　　处：《Science China Chemistry》2023年第11期3044-3053,共10页中国科学（化学英文版）

基　　金：supported by the start-up funding from Wuhan University;Fundamental Research Funds for the Central Universities(2042023kf0118)。

摘　　要：The mapping of DNA cytosine modifications is crucial for understanding the dynamic landscape of epigenetic regulation.While bisulfite sequencing has been the gold standard for decades,it suffers from limitations such as DNA degradation and low library quality due to harsh chemical treatment.In recent years,bisulfite-free methods have emerged as promising alternatives for detecting and quantifying DNA cytosine modifications.These methods employ enzymatic and chemical strategies to investigate cytosine modifications without the need for bisulfite treatment.This review provides an overview of the historical context of bisulfite-based methods and presents the current landscape of bisulfite-free methods.The advantages and limitations of each approach are discussed,along with insights into their applications.Furthermore,the review explores the existing challenges in the field and presents future perspectives and potential directions for advancing bisulfite-free mapping methods.The continued progress in bisulfite-free techniques holds great promise for unraveling the intricate nature of DNA cytosine modifications and their functional implications in diverse biological processes and diseases.

关 键 词：bisulfite-free cytosine modifications DNA sequencing base-resolution 

分 类 号：Q503[生物学—生物化学]