Microfluidic electrospray photo-crosslinkable κ-Carrageenan microparticles for wound healing  

作　　者：Zhiqiang Luo Junyi Che Lingyu Sun Lei Yang Yan Zu Huan Wang Yuanjin Zhao 

机构地区：[1]Department of Rheumatology and Immunology,Institute of Translational Medicine,The Affiliated Drum Tower Hospital,Nanjing University Medical School,Nanjing 210008,China [2]State Key Laboratory of Bioelectronics,School of Biological Science and Medical Engineering,Southeast University,Nanjing 210096,China [3]Oujiang Laboratory,Zhejiang Lab for Regenerative Medicine,Vision and Brain Health,Wenzhou,Zhejiang 325001,China [4]Wenzhou Institute,University of Chinese Academy of Sciences,Wenzhou,Zhejiang 325001,China [5]The Eighth Affiliated Hospital,Sun Yat-sen University,Shenzhen 518033,China

出　　处：《Engineered Regeneration》2021年第1期257-262,共6页再生工程（英文）

基　　金：supported by the National Key Research and Development Program of China(2020YFA0908200);the National Natural Science Foundation of China(52073060 and 61927805);the Guangdong Basic and Applied Basic Research Foundation(2019A1515011750);the Shenzhen Fundamental Research Program(JCYJ20190813152616459,JCYJ20210324133214038 and JCYJ20190808120405672).

摘　　要：When organs,especially external organs are injured,pathogens and excessive water loss can threaten the wound healing process and cause local irreversible tissue damage.Here,novel natural κ-Carrageenan microparticles(MPs)able to deliver bioactive proteins and small molecules to the wound surface for promoted regeneration were developed using microfluidic electrospray.The side chains of κ-Carrageenan were modified with methacrylate pendant groups to make the monomer photonic cross-linkable.By changing the concentration of methacrylated-κ-Carrageenan,different porosities of MPs with controllable drug loading were achieved.The size of the MPs can also be tailored by adjusting the voltage of the applied electric field.Vascular endothelial growth factor(VEGF)and antimicrobial peptides Eumenitin were co-loaded into the microparticles(VEGF-Eumenitin-MPs)and released sustainedly in phosphate-buffered saline.The VEGF-Eumenitin-MPs had high biocompatibility and intensive antibacterial activities for bacteria.Furthermore,the antibacterial and angiogenetic properties of the VEGF-Eumenitin-MPs were demonstrated in a infectious wound model and the chronic wound healing process was significantly enhanced in vivo.Therefore,the developed porous MPs with sustained drug release properties have the potential to serve as a platform to deliver bioactive proteins and small molecules to the wound surface for promoted regeneration.

关 键 词：MICROPARTICLE Hydrogel Microfluidics Wound healing VEGF Antimicrobial peptide 

分 类 号：O62[理学—有机化学]