m^(6)A mRNA modification potentiates Th17 functions to inflame autoimmunity  

作　　者：Xuefei Wang Chen Chen Hongwei Sun Kaiqiong Mao Jiameng Yao Weiqiao Zhang Meixiao Zhan Hua-Bing Li Zhiren Zhang Shu Zhu Ligong Lu 

机构地区：[1]Department of Geriatrics,Medical Center on Aging of Ruijin Hospital,Shanghai Jiao Tong University School of Medicine,Shanghai 200025,China [2]Shanghai Institute of Immunology,State Key Laboratory of Oncogenes and Related Genes,Shanghai Jiao Tong University School of Medicine,Shanghai 200025,China [3]Institute of Immunology,The CAS Key Laboratory of Innate Immunity and Chronic Disease,Division of Life Sciences and Medicine,University of Science and Technology of China,Hefei 230001,China [4]Institute of Health and Medicine,Hefei Comprehensive National Science Center,Hefei 230601,China [5]Guangdong Provincial Key Laboratory of Tumor Interventional Diagnosis and Treatment,Zhuhai Interventional Medical Center,Zhuhai People’s Hospital(Zhuhai Hospital Affiliated with Jinan University),Zhuhai 519000,China

出　　处：《Science China(Life Sciences)》2023年第11期2543-2552,共10页中国科学（生命科学英文版）

基　　金：supported by the National Natural Science Foundation of China(82230067,82061148013,91842105,81821001);Shanghai Science and Technology Committee(20JC1417400,201409005500);the National Key Research and Development Program of China(2018YFA0508000);the Strategic Priority Research Program of the Chinese Academy of Sciences(XDB29030101);the CAS Project for Young Scientists in Basic Research(YSBR-074);the Guangdong Provincial Key Laboratory of Tumor Interventional Diagnosis and Treatment(2021B1212040004).

摘　　要：N6-methyladenosine(m^(6)A),the most common and abundant epigenetic RNA modification,governs mRNA metabolism to determine cell differentiation,proliferation and response to stimulation.m^(6)A methyltransferase METTL3 has been reported to control T cell homeostasis and sustain the suppressive function of regulatory T cells(Tregs).However,the role of m^(6)A methyltransferase in other subtypes of T cells remains unknown.T helper cells 17(Th17)play a pivotal role in host defense and autoimmunity.Here,we found that the loss of METTL3 in T cells caused serious defect of Th17 cell differentiation,and impeded the development of experimental autoimmune encephalomyelitis(EAE).We generated Mettl3f/fIl17aCre mice and observed that METTL3 deficiency in Th17 cells significantly suppressed the development of EAE and displayed less Th17 cell infiltration into central nervous system(CNS).Importantly,we demonstrated that depletion of METTL3 attenuated IL-17A and CCR5 expression by facilitating SOCS3 mRNA stability in Th17 cells,leading to disrupted Th17 cell differentiation and infiltration,and eventually attenuating the process of EAE.Collectively,our results highlight that m^(6)A modification sustains Th17 cell function,which provides new insights into the regulatory network of Th17 cells,and also implies a potential therapeutic target for Th17 cell mediated autoimmune disease.

关 键 词：m^(6)A modification Th17 cells EAE SOCS family 

分 类 号：R593.2[医药卫生—内科学]