Distinct and shared endothermic strategies in the heat producing tissues of tuna and other teleosts  

作　　者：Baosheng Wu Xueli Gao Mingling Hu Jing Hu Tianming Lan Tingfeng Xue Wenjie Xu Chenglong Zhu Yuan Yuan Jiangmin Zheng Tao Qin Peidong Xin Ye Li Li Gong Chenguang Feng Shunping He Huan Liu Haimeng Li Qing Wang Zhenhua Ma Qiang Qiu Kun Wang 

机构地区：[1]School of Ecology and Environment,Northwestern Polytechnical University,Xi’an 710072,China [2]South China Sea Fisheries Research Institute,Chinese Academy of Fishery Sciences,Guangzhou 510300,China [3]State Key Laboratory of Agricultural Genomics,BGI-Shenzhen,Shenzhen 518083,China [4]BGI Life Science Joint Research Center,Northeast Forestry University,Harbin 150006,China [5]National Engineering Laboratory of Marine Germplasm Resources Exploration and Utilization,Marine Science and Technology College,Zhejiang Ocean University,Zhoushan 316022,China [6]Institute of Deep-Sea Science and Engineering,Chinese Academy of Sciences,Sanya 572000,China [7]The Key Laboratory of Aquatic Biodiversity and Conservation of Chinese Academy of Sciences,Institute of Hydrobiology,Chinese Academy of Sciences,Wuhan 430072,China [8]College of Life Sciences,University of Chinese Academy of Sciences,Beijing 100049,China

出　　处：《Science China(Life Sciences)》2023年第11期2629-2645,共17页中国科学（生命科学英文版）

基　　金：supported by the National Natural Science Foundation of China(32122021);the National Key Research and Development Program of China(2022YFC3400300);the 1000 Talent Project of Shaanxi Province to Q.Q.and K.W.,the Fundamental Research Funds of Northwestern Polytechnic University;the Open Foundation from Marine Sciences in the First-Class Subjects of Zhejiang(OFMS011)。

摘　　要：Although most fishes are ectothermic,some,including tuna and billfish,achieve endothermy through specialized heat producing tissues that are modified muscles.How these heat producing tissues evolved,and whether they share convergent molecular mechanisms,remain unresolved.Here,we generated a high-quality genome from the mackerel tuna(Euthynnus affinis)and investigated the heat producing tissues of this fish by single-nucleus and bulk RNA sequencing.Compared with other teleosts,tuna-specific genetic variation is strongly associated with muscle differentiation.Single-nucleus RNA-seq revealed a high proportion of specific slow skeletal muscle cell subtypes in the heat producing tissues of tuna.Marker genes of this cell subtype are associated with the relative sliding of actin and myosin,suggesting that tuna endothermy is mainly based on shivering thermogenesis.In contrast,cross-species transcriptome analysis indicated that endothermy in billfish relies mainly on nonshivering thermogenesis.Nevertheless,the heat producing tissues of the different species do share some tissue-specific genes,including vascular-related and mitochondrial genes.Overall,although tunas and billfishes differ in their thermogenic strategies,they share similar expression patterns in some respects,highlighting the complexity of convergent evolution.

关 键 词：ENDOTHERMY TUNA convergent evolution divergent strategies heat producing tissues 

分 类 号：Q953[生物学—动物学]