左旋咪唑和莫西菌素联合用药在小尾寒羊体内药代动力学研究  

Study on Pharmacokinetics of Combination of Levamisole and Moxidectin in Small-tailed Han Sheep

作　　者：张国华 刘雪松 金振华 张备 王丽坤 黄新育 薛沾枚 沈思思 杨昊天 陈国旺 海龙 ZHANG Guo-hua;LIU Xue-song;JIN Zhen-hua;ZHANG Bei;WANG Li-kun;HUANG Xin-yu;XUE Zhan-mei;SHEN Si-si;YANG Hao-tian;CHEN Guo-wang;HAI Long(Animal Husbandry and Veterinary Institute,Heilongjiang Academy of Agricultural Science,Qiqihar,Heilongjiang,161005,China)

机构地区：[1]黑龙江省农业科学院畜牧兽医分院,黑龙江齐齐哈尔161005

出　　处：《动物医学进展》2023年第11期59-65,共7页Progress In Veterinary Medicine

基　　金：国家肉羊产业技术体系项目(CARS-38);黑龙江省农科院创新工程项目(HNK2019CX16);2022年畜牧兽医分院自拟课题(ZNKT-202204);齐齐哈尔市创新激励项目(CNYGG-2022007)。

摘　　要：为了获得莫西菌素和左旋咪唑单独及联合用药在小尾寒羊体内的药物代谢动力学数据,并比较单独用药及联合用药时药动学参数差异,选择36只健康小尾寒羊,随机分为左旋咪唑皮下8 mg/kg组(LEVSC8)、莫西菌素皮下0.2 mg/kg组(MXDSC0.2)、联合用药组(LEVSC8+MXDSC0.2)皮下注射给药,每组12只小尾寒羊。给药后的不同时间段于颈静脉采集血液,离心获得血浆样本,通过高效液相色谱方法测定血浆中药物浓度,并通过Wlinnonlin软件获得药动学参数,通过统计学分析软件进行显著性差异比较。结果显示,左旋咪唑单独应用时,在小尾寒羊体内的达峰浓度为(1.79±0.69)μg/mL,达峰时间为(1.29±0.68)h,药时曲线下面积为(8.69±2.69)μg·h/mL,消除半衰期为(3.37±1.57)h;左旋咪唑和莫西菌素联合应用时,左旋咪唑在小尾寒羊体内的达峰浓度为(1.64±0.72)μg/mL,达峰时间为(1.35±0.73)h,药时曲线下面积为(8.03±2.69)μg·h/mL,消除半衰期为(3.69±1.71)h。左旋咪唑和莫西菌素联合用药时的药动学参数与左旋咪唑单独用药时相比,无显著性差异(P>0.05)。莫西菌素单独用药时,在小尾寒羊体内的达峰浓度为(40.57±10.06)ng/mL,达峰时间为(1.08±0.53)d,药时曲线下面积为(168.76±29.39)ng·d/mL,消除半衰期为(5.76±1.36)d;与左旋咪唑联合应用时,莫西菌素在小尾寒羊体内的达峰浓度为(39.89±13.49)ng/mL,达峰时间为(1.36±0.46)d,药时曲线下面积为(152.69±35.96)ng·d/mL,消除半衰期为(6.36±1.79)d。莫西菌素和左旋咪唑联合用药时的药动学参数与莫西菌素单独用药时,无显著性差异(P>0.05)。表明左旋咪唑和莫西菌素联合应用时,莫西菌素与左旋咪唑在小尾寒羊体内无动力学相互作用。研究结果可为左旋咪唑和莫西菌素在小尾寒羊上的联合应用提供依据。In order to obtain the pharmacokinetic data of moxidectin and levamisole in small-tailed Han sheep when used alone or in combination,and to compare the differences in pharmacokinetic parameters when used alone or in combination,36 healthy small-tailed Han sheep were selected and randomly divided into levamisole subcutaneously injected at a dose of 8 mg/kg group(LEV SC8),moxidectin subcutaneously injected at a dose of 0.2 mg/kg group(MXD SC0.2)and combined drug group(LEV SC8+MXD SC0.2).12 small-tailed Han sheep per group.Blood samples were collected from jugular vein at different times after administration.Plasma samples were obtained by centrifugation and drug concentration in plasma was determined by HPLC.The pharmacokinetic parameters were obtained by Wlinnonlin software and significant differences were compared by statistical analysis software.The results showed that when levamisole was used alone,the peak concentration in small-tailed Han sheep was(1.79±0.69)μg/mL,the peak time was(1.29±0.68)h,the area under the drug time curve was(8.69±2.69)μg·h/mL,and elimination half-life was(3.37±1.57)h;When levamisole and moxidectin were used together,the peak concentration of levamisole in small-tailed Han sheep was(1.64±0.72)μg/mL,the peak time was(1.35±0.73)h,the area under the drug time curve was(8.03±2.69)μg·h/mL,and the elimination half-life was(3.69±1.71)h.There was no significant difference between the pharmacokinetic parameters of levamisole combined with moxidectin and levamisole alone(P>0.05).When moxidectin was used alone,the peak concentration in small-tailed Han sheep was(40.57±10.06)ng/mL,the peak time was(1.08±0.53)d,the area under the drug time curve was(168.76±29.39)ng·d/mL,and the elimination half-life was(5.76±1.36)d.When levamisole and moxidectin were used together,the peak concentration of moxidectin in small-tailed Han sheep was(35.89±13.49)ng/mL,the peak time was(1.36±0.46)d,the area under the drug time curve was(152.69±35.96)ng·d/mL,and the elimination half-life was(6.36±1

关 键 词：左旋咪唑 莫细菌素 联合应用 药动学参数 

分 类 号：S859[农业科学—临床兽医学] S858.26[农业科学—兽医学]