银屑病皮损间充质干细胞来源外泌体对PBMCs分泌细胞因子的影响  

Effect of exosomes derived from mesenchymal stem cells in psoriatic lesions on cytokine secretion by PBMCs

作　　者：王思宇 牛旭平[2] WANG Siyu;NIU Xuping(Department of Dermatology,Ninth Clinical Medical College of Shanxi Medical University,Taiyuan 030001,China;Department of Dermatology,Taiyuan Central Hospital)

机构地区：[1]山西医科大学第九临床医学院皮肤科教研室,太原030001 [2]太原市中心医院皮肤科

出　　处：《山西医科大学学报》2023年第10期1368-1372,共5页Journal of Shanxi Medical University

基　　金：山西省卫生健康委科研课题项目(2022143)。

摘　　要：目的探究银屑病皮损及健康人皮肤间充质干细胞(dermal mesenchymal stem cells,DMSCs)来源外泌体(exosomes,EXO)对外周血单个核细胞(peripheral blood mononuclear cells,PBMCs)分泌细胞因子的影响。方法分离培养健康人皮肤组织及银屑病患者皮损处的间充质干细胞分泌的外泌体(DMSC-derived exosomes,DMSCs-EXO),从健康志愿者及银屑病患者外周血中分离培养PBMCs,分别加入不同来源的外泌体,于0,24,72 h收集上清液。ELISA法测定相关炎症因子IL-17、IL-23、IL-10和TGF-β1。结果ELISA结果显示,银屑病患者皮损处的间充质干细胞分泌来源的外泌体(psoriasis DMSC-derived exosomes,PDMSCs-EXO)干预后,健康人PBMCs中IL-17、IL-23上调(P<0.05),IL-10、TGF-β1下调(P<0.05);健康人皮肤处的间充质干细胞分泌来源的外泌体(healthy DMSC-derived exosomes,HDMSCs-EXO)干预后,银屑病患者PBMCs中IL-10、TGF-β1上调(P<0.05),IL-17、IL-23下调(P<0.05)。干预后24 h及72 h时,健康人PBMCs+PDMSCs-EXO组IL-17、IL-23高于健康人PBMCs+HDMSCs-EXO组(P<0.05),IL-10、TGF-β1低于健康人PBMCs+HDMSCs-EXO组(P<0.05);干预后24 h及72 h,银屑病患者PBMCs+HDMSCs-EXO组IL-17、IL-23低于银屑病患者PBMCs+PDMSCs-EXO组(P<0.05),干预后24 h,IL-10、TGF-β1高于银屑病患者PBMCs+PDMSCs-EXO组(P<0.05)。与银屑病患者PBMCs+PDMSCs-EXO组相比,健康人PBMCs+PDMSCs-EXO组在24 h时,IL-23、IL-17及TGF-β1无明显差异,在72 h时TGF-β1无明显差异;与健康人PBMCs+HDMSCS-EXO组相比,银屑病患者PBMCs+HDMSCS-EXO组在72 h时IL-23和IL-17水平无明显差异。结论PDMSCs-EXO可能影响PBMCs分泌促炎细胞因子并抑制抗炎细胞因子,同时HDMSCs-EXO参与调节细胞因子失衡引起的炎症反应。Objective To explore the effect of exosomes(EXO)derived from dermal mesenchymal stem cells(DMSCs)of both healthy skin and psoriasis lesions on the cytokine secretion patterns of peripheral blood mononuclear cells(PBMCs).Methods DMSC-derived exosomes(DMSCs-EXO)were extracted and cultured from skin tissues of healthy individuals and psoriasis lesions.Simultaneously,PBMCs were cultured from the peripheral blood of both healthy volunteers and individuals with psoriasis,and treated with different exosomes.Supernatants were collected at 0,24,72 h to detect the levels of inflammatory cytokines IL-17,IL-23,IL-10,and TGF-β1 by ELISA.Results ELISA results revealed that IL-17 and IL-23 levels were increased(P<0.05),and IL-10 and TGF-β1 levels were decreased in peripheral blood mononuclear cells(PBMCs)of healthy individuals after intervention with psoriatic dermal mesenchymal stem cell-derived exosomes(PDMSCs-EXO)from lesion sites in psoriasis patients(P<0.05).IL-17 and IL-23 levels were decreased(P<0.05),and IL-10 and TGF-β1 levels were increased in PBMCs of psoriasis patients after intervention with healthy dermal mesenchymal stem cell-derived exosomes(HDMSCs-EXO)(P<0.05).At 24 h and 72 h after intervention,IL-17 and IL-23 levels were higher in healthy PBMCs+PDMSCs-EXO group than in healthy PBMCs+HDMSCs-EXO group(P<0.05),while IL-10 and TGF-β1 levels were lower(P<0.05).IL-17 and IL-23 levels were lower at 24 h and 72 h after intervention in psoriasis PBMCs+PDMSCs-EXO group than in psoriasis PBMCs+HDMSCs-EXO group(P<0.05),while IL-10 and TGF-β1 levels were higher at 24 h(P<0.05).There was no significant difference in the levels of IL-23 and IL-17 at 24 h,and TGF-β1 at 24 h and 72 h between healthy PBMCs+PDMSCs-EXO group and psoriasis PBMCs+PDMSCs-EXO group.Similarly,no statistic differences were noted in IL-23 and IL-17 levels at 72 h between psoriasis PBMCs+HDMSCs-EXO group and healthy PBMCs+HDMSCs-EXO group.Conclusion PDMSCs-EXO can affect the pro-inflammatory cytokine secretion by PBMCs and inhibit the anti-inflamm

关 键 词：外泌体 银屑病 间充质干细胞 白细胞介素17 白细胞介素23 白细胞介素10 转化生长因子β1 

分 类 号：R751[医药卫生—皮肤病学与性病学]