烟草番茄环纹斑点病毒快速检测试纸条的研制及应用  

Development and application of test strips for rapid detection of tomato zonate spot virus in tobacco

作　　者：姜宁 夏振远[1] 卢灿华 黄昌军[1] 马俊红[1] 刘春明[2] 盖晓彤 JIANG Ning;XIA Zhenyuan;LU Canhua;HUANG Changjun;MA Junhong;LIU Chunming;GAI Xiaotong(Yunnan Academy of Tobacco Agricultural Sciences,Kunming 650021,China;Honghe Branch of Yunnan Provincial Tobacco Company,Mile 652399,Yunnan,China)

机构地区：[1]云南省烟草农业科学研究院,昆明市650021 [2]云南省烟草公司红河州公司,云南省652399

出　　处：《烟草科技》2023年第11期10-15,53,共7页Tobacco Science & Technology

基　　金：中国烟草总公司科技计划项目“基于传播途径阻断的烟草斑萎病绿色防控关键技术研究与应用”[110202001033(LS-02)];云南省烟草公司科技计划项目“云南省烟草病毒病调查及病毒检测技术研究”(2021530000242031);云南省烟草公司科技计划项目“烟草高风险农药残留分析及治理策略研究”(2021530000242030)。

摘　　要：为实现田间烟叶中番茄环纹斑点病毒(tomato zonate spot virus,TZSV)的快速检测,以胶体金标记特异性识别番茄环纹斑点病毒的单克隆抗体,制备了番茄环纹斑点病毒胶体金快速检测试纸条。结果表明,该试纸条可在5 min内完成田间烟叶的检测,与烟草上常见的病毒无交叉反应。试纸条检测烟草病毒汁液的灵敏度可达到稀释10000倍(质量体积比),田间发病烟叶的试纸条检测结果与RT-PCR检测结果吻合率大于96%。本研究中研制的番茄环纹斑点病毒快速检测试纸条具有操作简便、检测速度快、特异性强、灵敏度高、稳定性好等优点,适用于烟草田间番茄环纹斑点病毒的快速检测。To rapidly detect tomato zonate spot virus(TZSV)in tobacco fields,the monoclonal antibody against TZSV was labeled with colloidal gold,and rapid test strips based on the technique for detecting TZSV were developed.The results showed that the detection could be completed within 5 minutes without cross-reacting with other common viruses in tobacco.The visual detection limit of the test strip for TZSV was 10000-fold dilution of TZSV-infected tobacco leaf sap,and the agreement rate between the results obtained by the test strips and the RT-PCR test was over 96%.The developed test strip has the advantages of easy to use,fast detection,strong specificity,high sensitivity and good stability,and is thus suitable for rapid detection of TZSV in tobacco fields.

关 键 词：烟草 番茄环纹斑点病毒 单克隆抗体 胶体金试纸条 快速检测 

分 类 号：S432[农业科学—植物病理学]