中空高分子荧光微球的制备及其免疫层析检测应用  

Preparation of hollow polymer fluorescent microspheres and its application in lateral flow immunoassay

作　　者：洪正道 李振华[1] 徐明[2] 冯玮 李富友 HONG Zheng-Dao;LI Zhen-Hua;XU Ming;FENG Wei;LI Fu-You(Department of Chemistry,Fudan University,Shanghai 200438,China;Institute of Translational Medicine,Shanghai Jiao Tong University,Shanghai 200240,China)

机构地区：[1]复旦大学化学系,上海200438 [2]上海交通大学转化医学院,上海200240

出　　处：《无机化学学报》2023年第11期2055-2064,共10页Chinese Journal of Inorganic Chemistry

基　　金：国家重点研发计划“基础科研条件与重大科学仪器设备研发”专项资助项目基金(No.2022YFF0710000)资助。

摘　　要：以过硫酸钾(KPS)为引发剂,甲基丙烯酸缩水甘油酯(GMA)和苯乙烯(St)为单体,采用无皂乳液聚合法制备了表面官能团化的高分子微球。研究发现添加微量NaCl制备得到的高分子微球,经四氢呋喃(THF)溶胀处理后,能够得到单分散且表面完整的中空P(St-co-GMA)微球。随后使用有机溶胀的方式将Eu配合物染料负载于微球壳层中,实现了α1微球蛋白(α1-MG)和β2微球蛋白(β2-MG)的免疫层析检测。The functionalized polymer microspheres were prepared by soap-free emulsion polymerization using potassium persulfate(KPS)as the initiator,glycidyl methacrylate(GMA)and styrene(St)as monomers.It was found that microspheres prepared by adding a trace amount of NaCl could be swelled by tetrahydrofuran(THF)to obtain monodisperse hollow microspheres with complete surfaces.The Eu(Ⅲ)complex dye was then loaded into the microspheres shell by means of organic swelling,and immunochromatographic detection ofα1 microglobulin(α1-MG)andβ2 microglobulin(β2-MG)was performed.

关 键 词：Eu(Ⅲ)配合物 甲基丙烯酸缩水甘油酯 苯乙烯 中空微球 免疫层析 

分 类 号：O614.33[理学—无机化学]