竹节参多糖通过PI3K/AKT/NF-κB信号通路对急性肝损伤大鼠的影响  

Effect of Panax japonicus polysaccharide on acute liver injury rats via PI3K/AKT/NF-κB signaling pathway

作　　者：宋添力 唐浪 王一民 吴广阳 刘绪 黄胜 SONG Tianli;TANG Lang;WANG Yiming;WU Guangyang;LIU Xu;HUANG Sheng(Medical School,Hubei Minzu University,Enshi 445000,Hubei,China;Hubei Provincial Key Laboratory of Occurrence and Intervention of Rheumatic Diseases,Hubei Minzu University,Enshi 445000,Hubei,China;Hubei Enshi College,Enshi 445000,Hubei,China)

机构地区：[1]湖北民族大学医学部,湖北恩施445000 [2]湖北民族大学风湿性疾病发生与干预湖北省重点实验室,湖北恩施445000 [3]湖北恩施学院,湖北恩施445000

出　　处：《精细化工》2023年第11期2472-2479,2534,共9页Fine Chemicals

基　　金：湖北省自然科学基金青年项目(2019CFB358);国家自然科学基金青年基金项目(81801979)。

摘　　要：以竹节参(Panax japonicus)为原料,经水提醇沉法制备了竹节参多糖(PSPJ),通过观察和记录大鼠一般情况及肝脏指数(肝脏质量/体质量)探讨了PSPJ通过PI3K/AKT/NF-κB信号通路干预CCl4诱导的急性肝损伤(ALI)大鼠的作用机制;采用苏木素-伊红(H&E)染色法评价了PSPJ对肝组织病理变化的改善作用;采用免疫组织化学(IHC)染色检测了肝脏组织NF-κB的表达情况;通过蛋白免疫印迹(Westernblot)检测考察了PSPJ对ALI大鼠肝脏PI3K/AKT/NF-κB信号通路相关蛋白及炎症指标的表达水平。结果表明,与空白对照组相比,模型组的体质量、肝脏质量和肝脏指数均显著上升(P<0.05,表示差异有统计学意义,下同),模型组出现大量肝组织炎症浸润,用药组肝脏病理变化明显缓解;与空白对照组比较,模型组大鼠肝组织p-PI3K蛋白表达显著下降(P<0.01),PI3K、p-AKT、p-NF-κB、IL-1β蛋白表达显著升高(P<0.05);药物组p-PI3K蛋白表达显著上升(P<0.05),p-AKT、IL-1β蛋白表达显著下降(分别为P<0.01、P<0.05)。PSPJ通过PI3K/AKT/NF-κB通路信号中的相关关键蛋白表达减轻了CCl4诱导的大鼠ALI症状。Panax japonicus polysaccharide(PSPJ),prepared via water extraction and alcohol precipitation,was used for treatment of rats with CCl4-induced acute liver injury(ALI).The mechanism of PSPJ's intervention on the injury through PI3K/AKT/NF-κB signaling pathway was analyzed by observing and recording the general condition and liver index(liver mass/body mass)of rats.The effects of PSPJ on the pathological changes of liver tissue were evaluated by hematoxylin-eosin(H&E)staining,immunohistochemical(IHC)staining for the liver NF-κB expression,and protein immunoblotting(Western blot)analysis on the expression levels of PSPJ on PI3K/AKT/NF-κB signaling pathway related proteins and inflammatory indexes in ALI rats.The results showed that compared with the blank control group,the model group exhibited significant increment in the body mass,liver mass as well as liver index(P<0.05,indicating statistical significance,the same below),and a large number of liver tissue inflammatory infiltration,while the drug treated group displayed obvious relief in the liver pathological changes.Compared with the blank control group,the protein expression of p-PI3K in liver tissue of model group was significantly decreased(P<0.01),while the protein expressions of PI3K,p-AKT,p-NF-κB and IL-1βwere significantly increased(P<0.05).In drug treated group,the expression of P-PI3K protein was significantly increased(P<0.05),and the expression of p-AKT and IL-1βprotein was significantly decreased(P<0.01,P<0.05).Therefore,PSPJ alleviated CCl4-induced ALI symptoms in rats through the expression of related key proteins in the PI3K/AKT/NF-κB signaling pathway.

关 键 词：竹节参多糖 PI3K/AKT/NF-κB 急性肝损伤 抗氧化 抗炎 中药现代化技术 

分 类 号：R285.5[医药卫生—中药学]