Investigation of Two Precipitation Methods for Extracting Immunoglobulin Y(IgY)from Egg Yolks  

作　　者：Tran Thi Quynh Lan Tran Trong Kha 

机构地区：[1]Faculty of Animal Science and Veterinary Medicine,Nong Lam University-HCM city,6th Quarter,Linh Trung Ward,Thu Duc District,Ho Chi Minh city,Vietnam

出　　处：《Veterinary Science Research》2021年第2期23-30,共8页兽医科学研究（英文）

基　　金：the scientific research fund of Nong Lam University,Ho Chi Minh City for giving the grant to this study.

摘　　要：Two groups of hens(control and immunization group)were arranged in an experimental design with an immunization schedule of 3 injections of BSA antigen.IgY antibodies were extracted from egg yolks by two precipitation processes(chloroform and polyethylene glycol precipitates)and quantified using a standard curve of protein concentration.The purification of IgY was confirmed by SDS-PAGE.Total protein extracted from egg yoks were less contaminated with yellow pigments(lutein and zeaxanthin)nd by using chloroform precipitate.The 2 week post-immunization,IgY-1 concentration increased respectively to 3903±726μg.ml(chloroform-1 extraction process)and 2937±294μg.ml(PEG extraction process)(P<rd 0.01).After 3 immunization,IgY level obtaining from in immunization group extracted by chloroform process(6633±1166μg.ml-1)increased 2.7 times higher than that in control group(2482±414μg.ml-1).Whereas IgY concentrations obtained from PEG extraction process were not significantly different between the experimental group and control group.Chloroform and PEG precipitation methods had the same protein profile on the SDS-PAGE.IgY antibody was identified by the presence of bands corresponding with IgY heavy chain(67-70 kDa)and IgY light chain(25 kDa)for both precipitation processes.

关 键 词：Immunoglobulin Y Egg yolk PRECIPITATION Polyethylene glycol CHLOROFORM 

分 类 号：S85[农业科学—兽医学]