光照对贵州黄鸡ONECUT 1基因在不同产蛋期各组织中表达的影响  

Effects of Lighting on ONECUT1 Gene Expression in Different Tissues of Guizhou Yellow Chicken During Laying Period

作　　者：朱丽莉 杨波 田维荣 李亮[2] 李成军 李冬光[2] 伍革民[2] 张福平[4] ZHU Lili;YANG Bo;TIAN Weirong;LI Liang;LI Chengjun;LI Dongguang;WU Gemin;ZHANG Fuping(Guizhou Station of Grassland Technique and Experiment,Guiyang,Guizhou 550005;Guizhou Institute of Animal Husbandry and Veterinary Science,Guiyang,Guizhou 550005;Tongzi Center for Animal Disease Prevention and Control,Zunyi,Guizhou 563200;Guizhou University,Guiyang,Guizhou 550025,China)

机构地区：[1]贵州省草地技术试验推广站,贵州贵阳550005 [2]贵州省畜牧兽医研究所,贵州贵阳550005 [3]桐梓县动物疫病预防防控中心,贵州遵义563200 [4]贵州大学,贵州贵阳550025

出　　处：《贵州农业科学》2023年第11期49-54,共6页Guizhou Agricultural Sciences

基　　金：贵州省联合基金项目[黔科合LH字(2015)7067];贵州省科技厅科技支撑计划项目[黔科合支撑(2019)2288]。

摘　　要：【目的】分析光照对蛋鸡不同组织和不同产蛋时期ONECUT1基因的差异表达,探讨该基因对鸡产蛋性能的影响,为深入开展光照对鸡繁殖性能分子机理的研究奠定基础。【方法】选取贵州黄鸡体重相近的10周龄母鸡216只,在半舍饲养殖条件下(舍饲+运动场)随机分为自然光照组和早晚各增光1 h组,140日龄、200日龄、480日龄屠宰各4只,采集垂体、下丘脑、视网膜、输卵管和卵巢等组织,利用qRT-PCR技术检测ONECUT1基因的差异表达。【结果】ONECUT1基因在肌肉组织中表达量最高,输卵管、卵巢次之,肝脏最低。在垂体、下丘脑和卵巢组织中,ONECUT 1基因在产蛋高峰期(200日龄)的表达量显著高于开产日期(140日龄)和产蛋后期(480日龄)。垂体组织中200日龄的增光组显著高于自然光照组,输卵管中140日龄的自然光照组显著低于增光组。【结论】ONECUT1基因的表达具有时空特性,并受光照调节,与鸡产蛋性能有关。【Objective】The effect of lighting on differential expression of ONECUT1 gene in different tissue and different laying period of Guizhou yellow chicken was analyzed,and the effect of ONECUT1 gene on laying characteristics was explored,which laid a foundation for deep research on the molecular mechanism of light on chicken reproductive performance.【Method】A total of 21610-week-old Guizhou yellow chickens with similar weight were randomly divided into 2 groups.One group had been given natural light group,and another had been given 1 more hour of lighting in the morning and evening.4 hens with 140-day-old,4 hens with 200-day-old and 4 hens with 480-day-old were slaughtered,and the tissue of pituitary,hypothalami,retina,tubal and ovarian were collected,so as to detect the differential expression of ONECUT1 gene through qRT-PCR.【Result】ONECUT1 gene had the most expression in muscle tissue,followed by fallopian tubes and ovaries,and the lowest in liver.In pituitary,hypothalamic,and ovarian tissues,the expression of ONECUT1 gene at peak egg production period(200 days of age)was significantly higher than those at the start date(140 days of age)and late egg laying period(480 days of age).The 200-day-old light enhancement group in pituitary tissue was significantly higher than that in the natural light group,and the natural light group at 140 days old in the fallopian tubes was significantly lower than the light enhancement group.【Conclusion】The expressions of ONECUT1 gene was exhibiting temporal and spatial expression patterns and photoperiod regulated,which was related to laying performance.

关 键 词：蛋鸡 贵州黄鸡 光照周期 ONECUT1基因 差异表达 

分 类 号：S831.2[农业科学—畜牧学]