NaCl胁迫对番茄渗透调节物质含量及光合特性的影响  

Effects of NaCl Stress on Content of Osmotic Regulation Substance and Photosynthetic Characteristics of Tomato

作　　者：宋波[1] 李龙 文国琴[1] SONG Bo;LI Long;WEN Guoqin(College of Life Sciences,China West Normal University,Nanchong,Sichuan 637000,China)

机构地区：[1]西华师范大学生命科学学院,四川南充637000

出　　处：《贵州农业科学》2023年第11期85-91,共7页Guizhou Agricultural Sciences

基　　金：四川省教育厅项目“濒危植物绶草根际微生物调查与研究”(18ZB579)。

摘　　要：【目的】探明盐胁迫对番茄渗透调节物质及光合特性的影响,为其抗盐胁迫反应机理研究及抗盐品种选育奠定基础。【方法】以Micro-Tom番茄幼苗为材料,研究不同NaCl浓度(0 mmol/L、100 mmol/L、200 mmol/L、300 mmol/L、400 mmol/L和500 mmol/L)盐胁迫对番茄幼苗光合特性及幼苗叶片的可溶性糖、丙二醛、可溶性蛋白和脯氨酸含量的影响。【结果】盐胁迫下番茄幼苗叶片的可溶性糖和丙二醛含量随盐胁迫强度增加而升高,造成生理性干旱,损害叶片膜结构。盐胁迫导致PSII供体侧放氧复合体OEC受损,叶片对光能的捕获减少。PSII活性反应中心数量减少,最终PSII反应中心的热损耗升高。PSII受体侧PQ库减小,Q A向Q B的电子传递受到抑制,能量传递效率降低。【结论】盐胁迫损害番茄幼苗叶片膜结构,导致PSII供体侧放氧复合体OEC受损,减少叶片对光能的捕获能力。【Objective】The effects of NaCl stress on content of osmotic regulation substance and photosynthetic characteristics of tomato were studied,so as to lay the foundation for studying the response mechanism of tomato under salt stress and breeding of salt-resistant variety.【Method】Taking tomato‘Micro-Tom’as test material,the effects of different NaCl concentration(0 mmol/L,100 mmol/L,200 mmol/L,300 mmol/L,400 mmol/L and 500 mmol/L)on the photosynthetic characteristics,soluble sugar,MDA,soluble protein and proline of tomato seedlings were studied.【Result】Under the condition of NaCl stress,the soluble sugar and MDA content increased with the increase of salt stress intensity,which caused physiological drought and damaged membrane structure.Salt stress caused the damage of oxygen-evolving complex(OEC)on the donor side of PSII and the reduction of leaves’capture of light energy.The number of active reaction center of PSII reduced,so that the heat loss of PSII reaction center of increased.Plastoquinone(PQ)pool on the receptor side of PSII shrank under salt stress,then the electron transport of Q A to Q B was inhibited and the energy transfer efficiency reduced.【Conclusion】Salt stress damaged the membrane structure of leaves of tomato seedling,so that OEC on the donor side of PSII was injured and the capture ability of leaves to light was weakened.

关 键 词：番茄 盐胁迫 渗透调节物质 叶绿素荧光 PSII 光合作用 

分 类 号：S641.2[农业科学—蔬菜学]