SO_(3)^(2-)驱动自养短程反硝化工艺亚硝酸盐积累特性研究  

Nitrite accumulation characteristics in a SO_(3)^(2-)-driven partial autotrophic denitrification process

作　　者：聂裕婷 周鑫[1,2] 平彩霞 NIE Yu-ting;ZHOU Xin;PING Cai-xia(College of Environmental Science and Engineering,Taiyuan University of Technology,Jinzhong 030600,China;Innovation Center for Postgraduate Education in Municipal Engineering of Shanxi,Jingzhong 030600,China)

机构地区：[1]太原理工大学环境科学与工程学院,山西晋中030600 [2]山西省市政工程研究生教育创新中心,山西晋中030600

出　　处：《中国环境科学》2023年第11期5719-5727,共9页China Environmental Science

基　　金：国家自然科学基金资助项目(21607111);山西省基础研究计划项目(20210302123198)。

摘　　要：构建了一种亚硫酸盐驱动自养短程反硝化(SDAPD)新工艺旨在实现NO_(2)--N快速积累.采用厌氧序批式生物膜反应器,探究了不同进水硝酸盐浓度(25~250mg/L)及不同硫氮物质的量比(0.8、1.7、2.6)对亚硝酸盐积累特性的影响.结果表明,系统亚硝酸盐积累率(NAR)随进水硝酸盐浓度提高而增加.硫氮比能够显著影响NAR.S/N为1.7时,NAR最高达(64.7±3.0)%.周期实验表明:硝酸盐还原、亚硝酸盐积累同时伴随着亚硫酸盐去除及硫酸盐的生成.高S/N会促进胞外聚合物(EPS)蛋白质和多糖产生及PN/PS升高;三维荧光光谱显示色氨酸类物质是SDAPD系统中EPS的主要成分,其荧光峰、荧光强度与S/N密切相关.高通量测序发现Thiobacillus和Thermomonas是硫自养反硝化关键功能菌属.This study developed a novel process of sulfite-driven autotrophic partial denitrification(SDAPD)to achieve the rapid accumulation of NO_(2)--N.Anaerobic sequencing batch biofilm reactors were used to explore the effects on nitrite accumulation under different influent nitrate concentration(25~250mg/L)and different S/N molar ratios(0.8,1.7,2.6).The results show that nitrite accumulation rate(NAR)increased with the increase of influent nitrate concentration,and the S/N can significantly affect the NAR.The NAR was the highest up to(64.7%±3.0%)at S/N of 1.7.Periodic experiments show that nitrate reduction and nitrite accumulation were accompanied by sulfite removal and sulfate production.High S/N could promote the production of protein and polysaccharide in extracellular polymer(EPS)and increase the ratio of PN/PS.Three-dimensional fluorescence spectra shows that the tryptophan was the main component of EPS in SDAPD system,and its fluorescence peak and fluorescence intensity were closely related to S/N.High-throughput sequencing found that Thiobacillus and Thermomonas were key bacteria with the function of sulfur autotrophic denitrification.

关 键 词：亚硫酸盐 自养短程反硝化 亚硝酸盐积累 硫氮比 微生物群落 

分 类 号：X703[环境科学与工程—环境工程]