WDR38,a novel equatorial segment protein,interacts with the GTPase protein RAB19 and Golgi protein GM130 to play roles in acrosome biogenesis  

作　　者：Qiujie Gao Gang Liu Lihua Huang Yunfei Zhang Xinxing Zhang Xiaoyue Song Xiaowei Xing 

机构地区：[1]Center for Experimental Medicine,Third Xiangya Hospital,Central South University,Changsha 410013,China [2]Department of Laboratory Medicine,Third Xiangya Hospital,Central South University,Changsha 410013,China [3]The Institute of Reproduction and Stem Cell Engineering,School of Basic Medical Sciences,Central South University,Changsha 410078,China

出　　处：《Acta Biochimica et Biophysica Sinica》2023年第10期1561-1570,共10页生物化学与生物物理学报（英文版）

基　　金：supported by the grants from the Clinical Medical Technology Innovation Guidance Project of Hunan Province(No.2021SK53716);the Graduate Student Independent Exploration Innovation Fund of Central South University(No.1053320215836).

摘　　要：The WD40-repeat containing(WDR)proteins are enriched in the testis and play important roles in spermatogenesis.In the present study,we investigate the expression profile of WDR38,a novel member of the WDR protein family,in humans and mice.RT-qPCR(reverse transcription-quantitative polymerase chain reaction)results demonstrate that WDR38 mRNA is abundantly expressed in both the human and mouse testis.The expression of mouse Wdr38 is strictly regulated during development.Further immunofluorescence staining results show that WDR38 is located in the equatorial segment of the acrosome in human and mouse mature spermatozoa and is involved in acrosome biogenesis.Subcellular localization analysis reveals that the mouse Wdr38 protein is distributed in the perinuclear cytoplasm of transfected cells and colocalizes with the GTPase protein Rab19 and Golgi protein GM130.Coimmunoprecipitation(co-IP)assays demonstrate that Wdr38,Rab19 and GM130 interact with each other in the mouse testis and in HEK293T cells.In acrosome biogenesis,Wdr38,Rab19 and GM130 aggregate at the nuclear membrane to form large vesicles,and GM130 then detaches and moves towards the caudal region of the nucleus,whereas the Wdr38/Rab19 complex spreads along the dorsal nuclear edge and finally docks to the equatorial segment.These results indicate that WDR38 is a novel equatorial segment protein that interacts with the GTPase protein RAB19 and Golgi protein GM130 to play roles in acrosome biogenesis.

关 键 词：WDR38 RAB19 GM130 acrosome biogenesis equatorial segment 

分 类 号：Q51[生物学—生物化学]