CH02 peptide promotes ex vivo expansion of umbilical cord blood-derived CD34+hematopoietic stem/progenitor cells  

作　　者：Yiqi Yang Bihui Zhang Junye Xie Jingsheng Li Jia Liu Rongzhan Liu Linhao Zhang Jinting Zhang Zijian Su Fu Li Leisheng Zhang An Hong Xiaojia Chen 

机构地区：[1]Institute of Biomedicine&Department of Cell Biology,College of Life Science and Technology,Guangdong Province Key Laboratory of Bioengineering Medicine,Guangdong Provincial Biotechnology Drug&Engineering Technology Research Center/National Engineering Research Center of Genetic Medicine,Ji’nan University,Guangzhou 510632,China [2]The First Affiliated Hospital,Ji’nan University,Guangzhou 510630,China [3]Key Laboratory of Molecular Diagnostics and Precision Medicine for Surgical Oncology in Gansu Province&NHC Key Laboratory of Diagnosis and Therapy of Gastrointestinal Tumor,Gansu Provincial Hospital,Lanzhou 730000,China [4]Key Laboratory of Radiation Technology and Biophysics,Hefei Institute of Physical Science,Chinese Academy of Sciences,Hefei 230031,China

出　　处：《Acta Biochimica et Biophysica Sinica》2023年第10期1630-1639,共10页生物化学与生物物理学报（英文版）

基　　金：supported by the grants from the National Natural Science Foundation of China(Nos.81902801,82173729,82273833 and 82260031);the Program of Department of Natural Resources of Guangdong Province(No.GDNRC[2021]50);the Guangdong Provincial Key R&D Program(No.2022B1111070007);the Guangdong Basic and Applied Basic Research Foundation(No.2022A1515110620);the Science&Technology Program of Guangzhou City(No.20212210007);the Non-profit Central Research Institute Fund of Chinese Academy of Medical Sciences(No.2019PT320005);the 2021 Central-Guided Local Science and Technology Development Fund(No.ZYYDDFFZZJ-1).

摘　　要：Umbilical cord blood(UCB)is an advantageous source for hematopoietic stem/progenitor cell(HSPC)transplantation,yet the current strategies for large-scale and cost-effective UCB-HSPC preparation are still unavailable.To overcome these obstacles,we systematically evaluate the feasibility of our newly identified CH02 peptide for ex vivo expansion of CD34^(+)UCB-HSPCs.We herein report that the CH02 peptide is specifically enriched in HSPC proliferation via activating the FLT3 signaling.Notably,the CH02-based cocktails are adequate for boosting 12-fold ex vivo expansion of UCB-HSPCs.Meanwhile,CH02-preconditioned UCB-HSPCs manifest preferable efficacy upon wound healing in diabetic mice via bidirectional orchestration of proinflammatory and anti-inflammatory factors.Together,our data indicate the advantages of the CH02-based strategy for ex vivo expansion of CD34^(+)UCB-HSPCs,which will provide new strategies for further development of large-scale HSPC preparation for clinical purposes.

关 键 词：CH02 peptide umbilical cord blood CD34^(+)hematopoietic stem/progenitor cell Fms-like tyrosine kinase receptor 3 wound healing 

分 类 号：R457.7[医药卫生—治疗学]