紫白膏对大鼠皮肤肛周模型创面转化生长因子β_(1)、表皮生长因子及受体表达的影响  

Effect of Zibai Ointment on the expression of transforming growth factor-β_(1),epidermal growth factor and its receptor on the wounds of perianal model of rat skin

作　　者：黄娟[1] 石荣[1] 吴许雄[1] 李阳[1] 汪昭楚 HUANG Juan;SHI Rong;WU Xuxiong;LI Yang;WANG Zhaochu(Department of Proctology,People's Hospital Affiliated to Fujian University of Traditional Chinese Medicine,Fujian Province,Fuzhou350004,China;First School of Clinical Medicine,Fujian University of Traditional Chinese Medicine,Fujian Province,Fuzhou350004,China)

机构地区：[1]福建中医药大学附属人民医院肛肠科,福建福州350004 [2]福建中医药大学第一临床医学院,福建福州350004

出　　处：《中国当代医药》2023年第31期5-10,共6页China Modern Medicine

基　　金：中国民族医药学会科研项目(2019KYXM-Z1128-109);国家中医药管理局国医大师陈民藩传承工作室建设项目(国中医药办人教函〔2022〕245号);福建中医药大学校管科研课题(X2019060-学科)。

摘　　要：目的探讨紫白膏对大鼠皮肤肛周模型创面转化生长因子β_(1)(TGF-β_(1))、表皮生长因子(EGF)、表皮生长因子受体(EGFR)表达的影响。方法将54只清洁级SD大鼠随机分为空白组、模型组、阳性对照组、紫白膏组、大青叶组、莫匹罗星组各9只。分别于干预后3、7、14 d,记录各组的创面愈合率,并采用ELISA法检测TGF-β_(1)的表达,采用Western Blot法检测EGF、EGFR的表达。结果各组大鼠的创面愈合率干预14 d后高于本组干预7 d、干预7 d后高于本组干预3 d,差异有统计学意义(P<0.05)。紫白膏组的TGF-β_(1)表达量干预14 d后低于7 d、干预7 d后低于3 d,差异均有统计学意义(P<0.05)。干预14 d后,紫白膏组、大青叶组的创面愈合率高于模型组,差异有统计学意义(P<0.05)。干预后各时间点,模型组的TGF-β_(1)、EGF、EGFR表达量均高于空白组,差异有统计学意义(P<0.05)。干预3 d后,紫白膏组的TGF-β_(1)表达量高于空白组,低于模型组、阳性对照组;EGF表达水平高于空白组、模型组、大青叶组;EGFR表达量高于模型组,差异有统计学意义(P<0.05)。干预7 d后,紫白膏组的TGF-β_(1)表达量高于空白组,低于模型组、阳性对照组及莫匹罗星组;EGF表达量高于空白组、模型组,低于莫匹罗星组;EGFR表达量高于模型组、阳性对照组、大青叶组,差异有统计学意义(P<0.05)。干预14 d后,紫白膏组的TGF-β_(1)表达量低于模型组、阳性对照组,EGF表达水平高于空白组、模型组,EGFR表达水平高于模型组、大青叶组,差异有统计学意义(P<0.05)。结论紫白膏可能通过调控TGF-β_(1)的适当表达,促进EGF、EGFR的高表达,影响大鼠皮肤肛周模型的创面愈合。Objective To investigate the effect of Zibai Ointment on the expression of transforming growth factor-β_(1)(TGF-β_(1)),epidermal growth factor(EGF)and epidermal growth factor receptor(EGFR)on the wounds of perianal model of rat skin.Methods Fifty-four clean grade SD rats were randomly divided into blank group,model group,positive control group,Zibai Ointment group,Daqingye group and Mupirocin group,with 9 rats in each group.On 3,7,and 14 days after intervention,the wound healing rate of each group was recorded,and the expression of TGF-β_(1)was detected by ELISA method,and the expression of EGF and EGFR was detected by Western Blot method.Results The wound healing rate of each group after 14 days of intervention was higher than that of the same group after 7 days of intervention,and the wound healing rate after 7 days of intervention was higher than that of the same group after 3 days of intervention,and the differences were statistically significant(P<0.05).The expression of TGF-β_(1)in Zibai Ointment group after 14 days of intervention was lower than that after 7 days of intervention,and that after 7 days of intervention was lower than that after 3 days of intervention,with statistically significant differences(P<0.05).After 14 days of intervention,the wound healing rate of Zibai Ointment group and Daqingye group were higher than that of the model group,and the differences were statistically significant(P<0.05).At each time point after intervention,the expression levels of TGF-β_(1),EGF and EGFR in the model group were higher than those in the blank group,and the differences were statistically significant(P<0.05).After 3 days of intervention,the expression of TGF-β_(1)in Zibai Ointment group was higher than that in blank group,and lower than those in model group and positive control group,and the expression level of EGF was higher than those of blank group,model group and Daqingye group,and the expression of EGFR was higher than that of the model group,with statistically significant differences(P<0.05).Af

关 键 词：紫白膏 大鼠模型 创面愈合 转化生长因子β_(1) 表皮生长因子 表皮生长因子受体 

分 类 号：R657.1[医药卫生—外科学]