蛋白质组学分析染色体加倍对木薯表型及块根形成期代谢产物的影响  

Proteomics analysis of effects of chromosome doubling on cassava phenotype and metabolites during root formation

作　　者：陆柳英 阮孟斌[2] 吴正丹 施平丽 严华兵 曾文丹 肖亮 尚小红 曹升 LU Liuying;RUAN Mengbin;WU Zhengdan;SHI Pingli;YAN Huabing;ZENG Wendan;XIAO Liang;SHANG Xiaohong;CAO Sheng(Cash Crops Research Institute,Guangxi Academy of Agricultural Sciences,Nanning 530007,China;institute of Tropical Bioscience and Biotechnology,Chinese Academy of Tropical Agricultural Sciences,Haikou 571101,China)

机构地区：[1]广西壮族自治区农业科学院经济作物研究所,南宁530007 [2]中国热带农业科学院热带生物技术研究所,海口571101

出　　处：《植物生理学报》2023年第10期1870-1880,共11页Plant Physiology Journal

基　　金：广西自然科学基金面上项目(2020GXNSFAA259085);广西自然科学基金重点项目(2020GXNSFDA297031);中国博士后科学基金(2022MD723768);国家自然科学基金(32260445)。

摘　　要：多倍体育种是木薯种质创新的重要途径。为探讨木薯同源四倍体块根发育特性及其与二倍体的差异,本研究通过对木薯新品种‘新选048’(X048-2x)及其同源四倍体(X048-4x)进行表型鉴定、块根主要农艺性状测定、块根形成期主要代谢产物含量测定及蛋白质组分析。结果发现在块根形成期同源四倍体的平均单株块根数、最长块根长度均显著高于二倍体。同源四倍体薯肉中果糖和木质素含量均显著高于二倍体;筛选出的48个重要差异蛋白富集于细胞胞内,主要参与抗旱性、低温驯化等生物学过程,多数具有碳水化合物结合蛋白和多糖结合蛋白等分子功能。KEGG通路富集分析显示差异蛋白主要富集于苯丙酸生物合成通路,且同源四倍体可能是通过调控苯丙酸生物合成途径的上游介导了木质素积累,增加了块根形成的数量。本研究结果为解析木薯同源四倍体块根发育特性、代谢产物积累与蛋白调控机制奠定了理论基础。Polyploid breeding is an important approach to cassava(Manihot esculenta)germplasm innovation.In order to explore the difference between autotetraploid and diploid tuberous root development characteristics of cassava,the phenotypes and agronomic characters,metabolites and proteomics of autotetraploid(X048-4x)and diploid(X048-2x)of‘Xinxuan 048'variety were identified.It was found that the average number of tuberous roots per plant and the longest tuber length of X048-4x were higher than X048-2x significantly during root formation period.The contents of fructose and lignin in the flesh tubers of X048-4x were higher than those of X048-2x significantly.There were 48 important differential proteins between X048-4x and X048-2x,which were enriched in the intracellular cells and mainly involve in biological processes such as response to water deprivation,cold acclimation,and most proteins had molecular function such us carbohydrate binding and polysaccharide binding.KEGG pathway enrich analysis showed that the differential proteins were mainly enriched in phenylpropanoid biosynthesis pathway.And it was speculated that the autotetraploid cassava may mediate lignin accumulation by regulating the upstream pathway of phenylpropanoid biosynthesis,increase the numbers of tuberous root formed.The results provided a theoretical basis for analysising the mechanism of root development characteristics,metabolite accumulation and protein regulation mechanism in autotetraploid cassava.

关 键 词：木薯同源四倍体 蛋白组 农艺性状 块根发育 代谢产物 

分 类 号：S533[农业科学—作物学]