利用试管休眠芽进行芍药组培技术研究  

Study on tissue culture of Paeonia lactiflora by using test tube dormant buds

作　　者：李艳敏[1] 薛娴[2] 王慧娟[1] 高凯 高杰 袁欣 王利民[1] 符真珠[1] 张和臣[1] LI Yanmin;XUE Xian;WANG Huijuan;GAO Kai;GAO Jie;YUAN Xin;WANG Limin;FU Zhenzhu;ZHANG Hechen(Horticulture Institute of Henan Academy of Agricutural Sciences,Zhengzhou 450002,China;College of Agriculture,Henan University of Science and Technology,Luoyang,Henan 471003,China;Luoyang Academy of Agricultural and Forestry Sciences,Luoyang,Henan 471023,China)

机构地区：[1]河南省农业科学院园艺研究所,郑州450002 [2]河南科技大学农学院,河南洛阳471003 [3]洛阳市农林科学院,河南洛阳471023

出　　处：《植物生理学报》2023年第10期1943-1950,共8页Plant Physiology Journal

基　　金：河南省农业科学院基本科研业务费项目(2022ZC23和2023ZC029);河南省科技攻关项目(222102110406)。

摘　　要：本研究以芍药根茎芽为外植体进行组织培养技术研究,诱导其形成试管休眠芽,采用石蜡切片对休眠芽进行解剖结构观察,重点研究了不同浓度6-BA及蔗糖对休眠芽生长的影响,并对打破休眠的温度进行了筛选。结果表明,芍药试管休眠芽具有顶端分生组织、叶原基和腋芽原基,基部由表皮、薄壁细胞和胚性愈伤组织细胞团组成。6-BA和蔗糖显著影响试管休眠芽的形成和生长,适宜的休眠芽诱导培养基为WPM+0.4 mg·L^(-1)6-BA+60 g·L^(-1)蔗糖+6.5 g·L^(-1)琼脂,诱导率可以达到81.14%,增殖系数1.61。在8~12℃温度范围内,在不添加任何植物生长调节物质MS培养基中,试管休眠芽可以先生根后萌芽;其中,12℃培养条件下,试管休眠芽生根率为72.2%,萌芽率为83.3%。生根苗移栽成活后生长90 d左右,在根茎处形成新的休眠芽。In this study, the rhizome buds of Paeonia lactiflora were used as explants to conduct tissue culture technology research to induce them to form test tube dormant buds in vitro. Paraffin sections were used to observe the anatomical structure of dormant buds. The effects of different concentrations of 6-BA and sucrose on the growth of dormant buds were studied, and the dormancy-breaking temperature was screened.The results showed that the test tube dormant buds of P. lactiflora had apical meristem, leaf primordia and axillary bud primordia, and the base of dormant buds was callus that formed epidermis, parenchyma cells and embryogenic cell clusters. 6-BA and sucrose significantly affected the formation and growth of dormant buds in vitro, and the induction rate was 81.14%, proliferation coefficient was 1.61 when 6-BA(0.4 mg·L^(-1)) and sucrose(60 g·L^(-1)) were added to the WPM medium. In the temperature range of 8–12℃, the dormancy of test tube buds could be broken without adding any plants growth regulating substances in MS medium, and the dormant buds germinated after rooting. Under 12℃ condition, the rooting rate of dormant buds in test tubes was 72.2%, and the germination rate was 83.3%. The newly rooted seedlings transplanted from the roots took about 90 days to grow, and new dormant buds formed at the rhizome.

关 键 词：芍药 试管休眠芽 温度 生根 萌芽 

分 类 号：S682.12[农业科学—观赏园艺]