氢化镁调控的绿豆渗透耐性与一氧化氮介导的脯氨酸代谢有关  

Magnesium hydride regulates osmotic tolerance in mung bean via nitric oxide-induced proline metabolism

作　　者：张毅华 陆鈃 赵海懿 陈曦 王乔 冯宇 程晓晴 沈文飚[2] ZHANG Yihua;LU Xing;ZHAO Haiyi;CHEN Xi;WANG Qiao;FENG Yu;CHENG Xiaoqing;SHEN Wenbiao(College of Life Sciences,Shanxi Agricultural University,Jinzhong,Shanxi 030801,China;College of Life Sciences,Nanjing Agricultural University,Nanjing 210095,Chin)

机构地区：[1]山西农业大学生命科学学院,山西晋中030801 [2]南京农业大学生命科学学院,南京210095

出　　处：《植物生理学报》2023年第10期1964-1974,共11页Plant Physiology Journal

基　　金：国家自然科学基金(32100255);山西省基础研究计划(20210302124499);山西省高等学校科技创新项目(2021L133)。

摘　　要：氢气(H_(2))是一种新发现的气体信号分子,参与植物响应环境胁迫。目前,对H2的运用主要依赖于以电解法制备的富氢水,但因成本较高及操作复杂,此方法并不利于农业上大规模应用。氢化镁(MgH_(2))是一种固体储氢材料。近期,医学和植物学研究显示,MgH2可以作为释放H_(2)的供体,但是MgH2在调控植物干旱及渗透耐性方面的作用尚不明确。本研究表明,10 mg·L^(-1)MgH_(2)通过诱导一氧化氮(NO)爆发,显著缓解200 g·L^(-1)聚乙二醇(PEG)-6000对绿豆(Vigna radiate)幼苗造成的渗透胁迫。相比于单独PEG-6000胁迫,MgH_(2)预处理后的硝酸还原酶(NR)活性提高33.52%,Vr NR1和VrNR2基因表达水平分别提升93.60%和21.91%。通过测定脯氨酸含量、Δ1-吡咯啉-5-羧酸合成酶和脯氨酸脱氢酶活性以及相关编码基因表达水平,证实MgH2对绿豆幼苗渗透胁迫耐受性的调控与NO介导的脯氨酸生物合成有关。研究结果为MgH_(2)的农业应用提供初步的理论依据。As a newfound gasotransmitter,hydrogen gas(H2)participates in multiple environmental stress responses in plants.Currently,H2 application is mainly dependent on hydrogen-rich water(HRW),which is prepared by water electrolysis.However,the above method is not feasible for large-scale agricultural applications due to its high cost and complex operation.Magnesium hydride(MgH_(2))is a solid-state hydrogen source that has recently been shown to play a significant role in both human health and plant physiology,similar to H_(2).The regulation of MgH_(2)-induced plant tolerance against drought and osmotic stress remains unclear.In this study,200 g·L^(-1)polyethylene glycol(PEG)-6000-induced osmotic stress was significantly alleviated by 10 mg·L^(-1)MgH_(2)application in mung bean(Vigna radiate)seedling,and this effect was mediated by a burst of nitric oxide(NO).Compared with PEG-6000 stress alone,nitrate reductase(NR)activity was promoted by 33.52%,and expression levels of VrNR1 and VrNR2 were increased by 93.60%and 21.91%,respectively.Furthermore,MgH_(2)conferred osmotic tolerance in mung bean via NO-induced proline synthesis,which was supported by the increased proline content andΔ1-pyrroline-5-carboxylate synthetase activity,the decreased proline dehydrogenase activity,and corresponding transcripts.Taken together,our results provide a theoretical basis for the application of MgH_(2)in agriculture.

关 键 词：氢化镁 一氧化氮 脯氨酸 渗透胁迫 绿豆 

分 类 号：S522[农业科学—作物学]