粳糯稻抗稻瘟病基因检测与品种抗性鉴定  

Detection of Blast Resistance Genes and Identification of Resistance of Japonica Glutinous Rice Varieties

作　　者：邢运高 刘艳[1] 李健[1] 迟铭[1] 杨波[1] 李景芳 王宝祥[1] 徐大勇[1] XING Yungao;LIU Yan;LI Jian;CHI Ming;YANG Bo;LI Jingfang;WANG Baoxiang;XU Dayong(Lianyungang Agriculture Science Academy,Jiangsu Province/Jiangsu Collaborative Center for Modern Crop Production,Lianyungang,Jiangsu 222006)

机构地区：[1]江苏省连云港市农业科学院/江苏省现代作物生产协同创新中心,江苏连云港222006

出　　处：《核农学报》2023年第11期2134-2141,共8页Journal of Nuclear Agricultural Sciences

基　　金：国家现代农业产业技术体系建设专项(CARS-01);江苏省重点研发计划(现代农业)(BE2021321);江苏现代种业振兴“揭榜挂帅”项目(JBGS[2021]043);连云港市财政专项(QNJJ2214)。

摘　　要：为了探明糯稻的稻瘟病抗性水平和抗稻瘟病基因分布,评价抗稻瘟病基因的抗性效应,利用自然诱发鉴定与人工接种鉴定相结合的方法鉴定65个糯稻品种(品系)的稻瘟病抗性,利用竞争性等位基因特异性PCR(KASP)标记技术检测Pi2、Pi9、Pigm、Pi1、Pikh、Pik、Pi5、Pita等8个基因。2021年自然诱发鉴定、2021—2022年人工接种鉴定中抗以上品种(品系)的比例分别是41.6%、24.6%和6.2%,说明糯稻品种(品系)的稻瘟病抗性年际间波动大、整体抗病性一般。除了Pigm外,其他7个抗病基因都有分布,分布频率差异较大,Pita基因在供试材料中分布频率最高,达到了67.7%,其次是Pikh、Pi9、Pi2、Pi5、Pik、Pi1,分布频率为47.7%、38.5%、27.7%、13.8%、4.6%、1.5%。供试糯稻品种(品系)携带0~4个抗病基因不等,仅有连糯19105携带4个抗性基因。国审、江苏审定的品种携带Pita的比例最高,安徽审定品种携带Pi9的比例最高,山东审定品种携带Pi5的比例最高,但不携带Pi2基因,Pik基因仅存在于国审及江苏审的品种中。携带3个抗性基因的供试品种(品系)抗性最好,其中“Pi2+Pita+Pik”、“Pi9+Pita+Pik”连续两年的自然诱发鉴定和人工接种鉴定抗病品种(品系)的比例均达到100%,年际间抗病稳定,可作为改良粳糯稻瘟病抗性主要的基因组合。本研究结果对粳糯稻的抗稻瘟病改良提供了理论依据。In order to ascertain the resistance to rice blast and the distribution of rice blast resistance genes,evaluate the effects of rice blast resistance genes,65 glutinous rice varieties(lines)were used to identify their resistance to rice blast by the combination of natural induction identification and artificial inoculation identification.The genotypes were analyzed with the kompetitive allele-specific PCR(KASP)markers of Pi2,Pi9,Pigm,Pi1,Pikh,Pik,Pi5 and Pita.The proportion of varieties with resistance to rice blast identified by natural induction in 2021 and by artificial inoculation in 2021-2022 were 41.6%,24.6%and 6.2%respectively.It showed that the resistance of glutinous rice varieties(lines)to rice blast fluctuated greatly between years,and the overall resistance was ordinary.In addition to Pigm,other seven disease resistance genes were all distributed in these rice lines,with a large difference in distribution frequency.Pita gene had the highest distribution frequency among the tested materials,reaching 67.7%,followed by Pikh,Pi9,Pi2,Pi5,Pik,Pi1 genes with the frequency of 47.7%,38.5%,27.7%,13.8%,4.6%,1.5%,respectively.The tested glutinous rice varieties carried 0-4 resistance genes,and only Liannuo 19105 carried 4 resistance genes.The varieties approved by the National and Jiangsu carried the highest proportion of Pita,the varieties approved by Anhui carried the highest proportion of Pi9,and the varieties approved by Shandong carried the highest proportion of Pi5,but do not carry Pi2 gene.Pik gene only existed in varieties approved by the National and Jiangsu.The tested varieties carrying three resistance genes had the best resistance,among which‘Pi2+Pita+Pik’,‘Pi9+Pita+Pik’showed 100%resistance by natural induction and artificial inoculation for two consecutive years,and the disease resistance was stable between years,which could be used as the main genes combination for improving the blast resistance of japonica glutinous rice.The results of this study provided a basis for disease resistance impr

关 键 词：糯稻 稻瘟病 KASP 抗性分析 

分 类 号：S435.111.41[农业科学—农业昆虫与害虫防治]