毛竹PheFT12a基因过表达对拟南芥开花及芽发育的影响  

Effects of Overexpression of the Moso Bamboo PheFT12a on Flowering and Bud Development of Arabidopsis

作　　者：张雨佳 刘丽[1] 邹龙海 周敏舒 畅欣 郭小勤[1] ZHANG Yujia;LIU Li;ZOU Longhai;ZHOU Minshu;CHANG Xin;GUO Xiaoqin(State Key Laboratory of Subtropical Silviculture/Bamboo Research Institute,Zhejiang A&F University,Hangzhou,Zhejiang 311300)

机构地区：[1]浙江农林大学,省部共建亚热带森林培育国家重点实验室/竹子研究院,浙江杭州311300

出　　处：《核农学报》2023年第11期2142-2150,共9页Journal of Nuclear Agricultural Sciences

基　　金：浙江省自然科学基金重点项目(LZ21C160001);国家自然科学基金(32171880)。

摘　　要：FLOWERING LOCUS T(FT)亚家族在植物生长发育过程中发挥着重要作用。为探究毛竹FT基因的表达特性及生物学功能,本研究通过反转录PCR(RT-PCR)从毛竹中克隆到1个FT同源基因PheFT12a,该基因编码区序列长度为522 bp,包含典型的4个外显子和3个内含子,编码173个氨基酸,编码蛋白包含完整的磷脂酰乙醇胺结合蛋白(PEBP)结构域。系统进化树分析结果显示,PheFT12a与水稻(Oryza sativa)OsFTL12的亲缘关系最近,与OsFTL2(Hd3a)和拟南芥(Arabidopsis thaliana)FT的亲缘关系相对较远。实时荧光定量PCR(qRT-PCR)结果显示,PheFT12a基因在毛竹实生苗叶片中表达最高,茎次之。亚细胞定位结果显示,PheFT12a蛋白定位于细胞核和细胞质,主要富集于细胞核。转化拟南芥ft突变体表型结果显示,PheFT12a能明显回补ft突变体的晚花表型,可提高回补植株的主茎数和侧枝数。本研究为进一步分析PheFT12a的生物学功能提供了参考依据,并为毛竹开花及芽发育的分子机制提供了基础资料。FLOWERING LOCUS T(FT)subfamily plays an important role in plant growth and development.In order to explore the expression characteristics and biological functions of FT gene in moso bamboo,a FT homologous gene PheFT12a was obtained by reverse transcription-polymerase chain reaction(RT-PCR).The coding sequence(CDS)of PheFT12a was 522 bp in length,contained four exons and three introns,and encoded 173 amino acids.The encoded protein contained a complete phosphatidyl ethanolamine-binding protein(PEBP)domain.Phylogenetic tree analysis showed that PheFT12a had the closest relationship with OsFTL12 in rice(Oryza sativa),but had a relatively distant relationship with OsFTL2(Heading date 3a,Hd3a)and AtFT.The quantitative real-time PCR(qRT-PCR)results showed that the expression of PheFT12a was the highest in leaves of moso bamboo seedlings,followed by stems.Subcellular localization assay showed that PheFT12a protein was located in the nucleus and cytoplasm,mainly enriched in the nucleus.The phenotype of Arabidopsis ft mutant transformed with 35S∷PheFT12a showed that PheFT12a could complement the late flowering phenotype of ft mutant and increased the number of stems and lateral branches.This study provides a reference for further analysis of the biological function of PheFT12a,and provides basic information for the molecular mechanism of bamboo flowering and shoot development.

关 键 词：毛竹 FLOWERING LOCUS T(FT)基因 PEBP结构域 开花 芽发育 

分 类 号：S795[农业科学—林木遗传育种]