盐爪爪根际产ACC脱氨酶菌株筛选及促生特性研究  

Isolation of ACC Deaminase Producing Strains From the Rhizosphere Soil of Kalidium foliatum and Identification of Their Growth-Promoting Characteristics

作　　者：李明源 王继莲 田世梅 LI Mingyuan;WANG Jilian;TIAN Shimei(Department of Biologic and Geographic Sciences,Kashi University/Key Laboratory of Biological Resources and Ecology of Pamirs Plateau in Xinjiang Uygur Autonomous Region,Kashi,Xinjiang 844000)

机构地区：[1]喀什大学生命与地理科学学院/新疆帕米尔高原生物资源与生态重点实验室,新疆喀什844000

出　　处：《核农学报》2023年第11期2151-2157,共7页Journal of Nuclear Agricultural Sciences

基　　金：国家自然科学基金(32160408);新疆维吾尔自治区自然科学基金项目(2021D01A18)。

摘　　要：为探究盐碱区生态环境改良方式,丰富产1-氨基环丙烷-1-羧酸(ACC)脱氨酶菌种资源,从新疆克孜勒苏柯尔克孜自治州盐碱地盐爪爪(Kalidium foliatum)根际土壤中筛选产ACC脱氨酶菌株,结合16S rRNA基因测序对优良菌株鉴定,通过盆栽接种试验验证其促生效果。结果从盐爪爪根际分离到26株产ACC脱氨酶菌株,酶活性为0.4~5.6 U·mg^(-1),以PM14、PM16和PM24活性最高。经鉴定,菌株PM14、PM16和PM24分别归属肠杆菌属(Enterobacter)、赖氨酸芽孢杆菌属(Lysinibacillus)和假单胞菌属(Pseudomonas)。盆栽试验表明,接种3株菌对非盐碱胁迫下拟南芥地上(株高、地上干重)和地下(根长、根干重)部分生长均有明显促进作用;对盐碱土栽培小麦的地上部分(株高、茎粗、鲜重和干重、叶绿素含量)和根干重也有积极影响,尤以PM16最显著。PM14和PM16兼具解磷、固氮和分泌吲哚-3-乙酸(indole-3-acetic acid, IAA)的能力,PM24兼具固氮和分泌IAA的能力,均具有多种促生功能。本研究结果为盐碱区微生物菌肥的开发提供了菌种资源和理论支持。To enrich the resources of strains which can produce 1-aminocyclopropane-1-carboxylate(ACC)deaminase and to explore the ecological environment improvement methods in saline-alkali land,strains producing high ACC deaminase production ability were isolated from the rhizosphere soil of Kalidium foliatum in Kizilsu Kirghiz Autonomous Prefecture,Xinjiang.The isolates were identified according to 16S rRNA gene identification,and their growth-promoting effects on plant seedlings were verified.As a result,a total of 26 isolates were screened,and their enzyme activity were 0.4-5.6 U·mg^(-1),with the highest activity in strains PM14,PM16 and PM24.16S rRNA gene sequencing showed that strains PM14,PM16,and PM24 belonged to genus Enterobacter,Lysinibacillus,and Pseudomonas,respectively.The seed pot experiments showed that the ACC deaminase producing strains had a significant promoting effect on both the development of aboveground(plant height,aboveground dry weight)and underground parts(root length,root dry weight)of Arabidopsis thaliana under non-saline stress.And the strains could also improve the development of aboveground part(plant height,stem diameter,fresh weight,shoot dry weight,and chlorophyll content)and underground part(root dry weight)of wheat seedlings under saline-alkali stress,especially the strain PM16.In addition,strains PM14 and PM16 had the ability of phosphorus solubilization,nitrogen fixation,and indole-3-acetic acid(IAA)production,whereas strain PM24 had the ability of nitrogen fixation,all belong to multifunctional strains.This study provided strain resources and theoretical support for the development of microbial fertilizer in saline-alkali land.

关 键 词：盐碱区 盐爪爪 ACC脱氨酶 根际 促生效果 

分 类 号：S144[农业科学—肥料学]