Lanthanide-based bulky counterions against aggregation-caused quenching of dyes in fluorescent polymeric nanoparticles  

作　　者：Caterina Severi Satu Lahtinen Jaana Rosenberg Andreas Reisch Tero Soukka Andrey S.Klymchenko 

机构地区：[1]Laboratoire de Biophotonique et Pathologies,Faculte de Pharmacie,UMR 7021 CNRS,Universite de Strasbourg,Cedex,Illkirch,France [2]Department of Biotechnology,University of Turku,Turku,Finland

出　　处：《Aggregate》2022年第1期168-177,共10页聚集体（英文）

基　　金：European Research Council ERC Consolidator grant Bright Sens,Grant/Award Number:648528。

摘　　要：Dye-loaded polymeric nanoparticles(NPs)are promising bioimaging agents because of their available surface chemistry,high brightness,and tunable optical properties.However,high dye loadings can cause the aggregation-caused quenching(ACQ)of the encapsulated fluorophores.Previously,we proposed to mitigate the ACQ inside polymeric NPs by insulating cationic dyes with bulky hydrophobic counterions.In order to implement new functionalities into dye-loaded NPs,here,we extend the concept of bulky counterions to anionic lanthanide-based complexes.We show that by employing Gd-based counterions with octadecyl rhodamine B loaded NPs at 30 wt% versus polymer,the fluorescence quantum yield can be increased to 10-fold(to 0.34).Moreover,Gd-anion provides NPs with enhanced contrast in electron microscopy.A combination of a luminescent Eu-based counterion with a far-red to near-infrared cyanine 5 dye(DiD)yields Forster resonance energy transfer NPs,where the UV-excited Eu-based counterion transfers energy to DiD,generating delayed fluorescence and large stokes shift of -340 nm.Cellular studies reveal low cytotoxicity of NPs and their capacity to internalize without detectable dye leakage,in contrast to leaky NPs with small counterions.Our findings show that the aggregation behavior of cationic dyes in the polymeric NPs can be controlled by bulky lanthanide anions,which will help in developing bright luminescent multifunctional nanomaterials.

关 键 词：aggregation-caused quenching dye-loaded polymeric nanoparticles fluorescence microscopy 

分 类 号：O63[理学—高分子化学]