基于UPLC-Q-Exactive和HPLC-PDA的青钱柳叶总黄酮化学成分的鉴定分析  

Analysis of Main Active Components from the Leaves of Cyclocarya Paliurus byUPLC-Q-Exactive and HPLC-PDA

作　　者：刘雅婷 罗春花 李鑫[2] 黄立滢 陈卫卫[2] Liu Yating;Luo Chunhua;Li Xin;Huang Liying;Chen Weiwei(Jiangsu Health Vocational College,Nanjing 211800;Guangxi University of Chinese Medicine,Nanning 530200,China)

机构地区：[1]江苏卫生健康职业学院,江苏南京211800 [2]广西中医药大学,广西南宁530200

出　　处：《广东化工》2023年第22期139-144,共6页Guangdong Chemical Industry

基　　金：江苏卫生健康职业学院院级科研项目(JKD2021003);国家自然科学基金项目(82000572);江苏省自然科学基金项目(BK20200056);广西中医药大学2018年博士科研启动基金项目(2018BS029);广西壮瑶药重点实验室课题(GXZYZZ2019-13)。

摘　　要：目的:基于UPLC-Q-Exactive和HPLC-PDA技术定性鉴别青钱柳叶总黄酮中的化学成分。方法:通过UPLC-Q-Exactive技术初步分析青钱柳叶总黄酮部位的化学成分,采用HPLC-PDA技术将其中与保肝作用相关的7种成分与对照品进行比对,进一步确证。结果:初步鉴定出青钱柳叶总黄酮中7大类共44种化学成分,其中黄酮类21种,并进一步确证了异槲皮苷、鞣花酸、槲皮素、异绿原酸C、山奈酚、金丝桃苷、绿原酸7种化学成分。结论:本文建立的方法可为青钱柳叶总黄酮的质量评价和控制提供依据。Objective:UPLC-Q-Exactive and HPLC-PDA techniques were used to identify the chemical constituents of total flavonoids from Cyclocarya paliurus leaves.Methods:The chemical components of otal flavonoids from Cyclocarya paliurus leaves were analyzed by UPLC-Q-Exactive technique,and then the 7 components related to anti-liver fibrosis were compared with the control substances by HPLC-PDA method to further confirm.Results:A total of 44 chemical components were identified from 7 categories of flavonoids,including 21 flavonoids.Seven chemical components of isoquercetin,ellagic acid,quercetin,isochlorogenic acid C,kaanneferol,hypericin and chlorogenic acid were further identified.Conclusion:The method established in this paper can provide a reference for the quality evaluation and control of total flavonoids from Cyclocarya paliurus leaves.

关 键 词：青钱柳叶总黄酮 UPLC-Q-Exactive HPLC-PDA 化学成分 定性鉴别 

分 类 号：O657[理学—分析化学] R917[理学—化学]