25-羟基维生素D_(3)对肉鸡生长性能、骨骼发育及小肠磷转运蛋白基因表达的影响  

Effects of 25-hydroxycholecalciferol on Growth Performance,Bone Development and Phosphate Transporter Gene Expressions in the Small Intestine of Broiler Chickens

作　　者：何蕾 段延民 张宁 齐雯露[2,3] 贺明星 曹金党 韩进诚 HE Lei;DUAN Yanmin;ZHANG Ning;QI Wenlu;HE Mingxing;CAO Jindang;HAN Jincheng(College of Life Sciences,Henan Normal University,Henan 453000,China;College of Biology and Food,Shangqiu Normal University,Henan 476000,China;Henan Engineering Research Center of Green Feed Additive Development and Application,Henan 476000,China;Shaanxi Academy of Modern Agricultural Sciences,xi'an Shaanxi 710018,China;College of Animal Science and Technology,Henan Agricultural University,Henan 450046,China;Henan Zhengbenqingyuan Technology Development Co.Ltd,Henan 476000,China;Shandong Haineng Bio-engineering Co.Ltd,Shandong 276800,China)

机构地区：[1]河南师范大学生命科学学院,河南新乡453000 [2]商丘师范学院生物与食品学院,河南商丘476000 [3]河南省绿色功能饲料添加剂开发与应用工程技术研究中心,河南商丘476000 [4]陕西省现代农业科学研究院,陕西西安710018 [5]河南农业大学动物科技学院,河南郑州450046 [6]河南正本清源科技发展股份有限公司,河南商丘476000 [7]山东海能生物工程有限公司,山东日照276800

出　　处：《家畜生态学报》2023年第11期24-28,共5页Journal of Domestic Animal Ecology

基　　金：国家自然科学基金项目(32072753,U1704107)。

摘　　要：试验旨在探究饲粮添加2000 IU/kg 25-羟基维生素D_(3)(25-OH-D_(3))对1~14日龄罗斯308肉鸡生长性能、骨骼发育及十二指肠细胞核维生素D受体(nVDR)、细胞膜维生素D受体(mVDR)和IIb型磷转运蛋白(NaPi-IIb)基因表达的影响。选择140只健康、体重相近的1日龄罗斯308肉鸡,随机分为2个处理,每组5个重复,每重复14只。设计2种饲粮,在基础饲粮(不含维生素D)中添加0、2000 IU/kg的25-OH-D_(3),饲喂粉状饲料,肉鸡自由采食,保证饮水充足,试验期为14 d。结果表明:(1)与基础饲粮组相比,饲粮添加2000 IU/kg 25-OH-D_(3)能显著提高1~14日龄罗斯308肉鸡采食量和体增重,显著降低料重比(P<0.05)。(2)与基础饲粮组相比,饲粮添加2000 IU/kg 25-OH-D_(3)显著提高1~14日龄肉鸡胫骨重量、长度、灰分含量、灰分重量、磷含量、钙含量(P<0.05),但没有影响肉鸡胫骨直径(P>0.05)。(3)与基础饲粮组相比,添加2000 IU/kg 25-OH-D_(3)显著提高了1~14日龄罗斯308肉鸡十二指肠mVDR、nVDR和NaPi-IIb mRNA表达量(P<0.05)。试验结果表明,添加25-OH-D_(3)可增加1~14日龄肉鸡十二指肠mVDR、nVDR和NaPi-IIb mRNA基因的表达水平,促进小肠磷吸收,改善肉鸡生长性能和骨骼发育。This study was conducted to investigate the effects of adding 2000IU/kg 25-hydroxycholecalciferol(25-OH-D_(3))on growth performance,bone development and gene expressions of nuclear vitamin D receptor(nVDR),membrane vitamin D receptor(mVDR),and type IIb phosphate transporter in the duodenum of Ross 308 broilers from 1 to 14 days of age.A total of 140 Ross 308 broilers were randomly divided into 2 treatments with 5 replicates per treatment and 14 broilers per replicate.Two levels of 25-OH-D_(3)(0 and 2000 IU/kg)were added to the basal diet(without supplementing vitamin D).Broilers were given access to mash feed and water ad libitum during the experiment.The test lasted for 14 days.Results showed that the addition that of 2000 IU/kg 25-OH-D_(3) significantly increased the body weight gain and feed intake of broilers from 1 to 14 days of age,and it decreased the feed conversion ratio of broilers(P<0.05).The addition of 2000 IU/kg 25-OH-D_(3) increased the weight,length,ash weight,ash content,calcium content,and phosphorus content of tibia in broilers from 1 to 14 days of age(P<0.05).25-OH-D_(3) did not affect tibia diameter of broilers(P>0.05).Compared with the basal diet,the addition of 2000 IU/kg 25-OH-D_(3) significantly increased the mRNA expression levels of nVDR,mVDR and NaPi-IIb in the duodenum of broilers(P<0.05).These data indicated that the addition of 25-OH-D_(3) can increase the mRNA expression levels of nVDR,mVDR and NaPi-IIb in the duodenum,promote the absorption of phosphorus in the small intestine,and improve growth performance and bone development of broilers.

关 键 词：25-羟基维生素D_(3) 磷转运蛋白 肉鸡 生长性能 骨骼质量 

分 类 号：S831.5[农业科学—畜牧学]