辣木叶水提物对油酸钠-钠棕榈酸酯诱导的HepG2细胞脂质代谢及抗氧化能力的影响  

Effects of Moringa oleifera leaf aqueous extract on lipid metabolism and antioxidant capacity in HepG2 cells induced by sodium oleate-sodium palmitate

作　　者：伍卫红 黄燕秋 李嘉俊 敬美莲 黄永昌 杨卓凡 张子恒 燕宇轩 宣攒威 胡巨豪 杨舒乔 周红祖[3] 余惠旻 WU Weihong;HUANG Yanqiu;LI Jiajun;JING Meilian;HUANG Yongchang;YANG Zhuofan;ZHANG Ziheng;YAN Yuxuan;XUAN Zanwei;HU Juhao;YANG Shuqiao;ZHOU Hongzu;YU Huimin(Guangdong Jiangmen Chinese Medicine College,Jiangmen 529000,China;School of Medicine,Shenzhen University,Shenzhen 518060,China;Shenzhen Chinese Medicine Hospital,Shenzhen 518020,China)

机构地区：[1]广东江门中医药职业学院,广东江门529000 [2]深圳大学医学部,广东深圳518060 [3]深圳市中医院,广东深圳518020

出　　处：《药物评价研究》2023年第10期2152-2158,共7页Drug Evaluation Research

基　　金：广东省教育厅普通高校认定类科研项目(2020KTSCX342);深圳市药学会医院药学研究基金(恒瑞基金)(SZ2022A25);深圳市基础研究面上项目(JCYJ20220531102208019);深圳大学2023年度聚徒项目(聚徒+学术)。

摘　　要：目的探讨辣木叶水提物(MOLAE)对脂肪堆积人HepG2细胞的调节作用。方法制备MOLAE冻干粉;通过CCK-8法检测油酸钠-钠棕榈酸酯(O-P)对HepG2细胞活力的影响、油红O染色检测O-P对细胞脂质堆积的影响、试剂盒法检测O-P对细胞三酰甘油(TG)、总胆固醇(TC)水平的影响,筛选O-P诱导HepG2细胞脂质代谢异常模型的作用浓度及时间;CCK-8法检测辛伐他汀、MOLAE对HepG2细胞活力的影响,筛选安全作用浓度;设立对照组、模型组、辛伐他汀(阳性药,15μmol·L^(−1))组和MOLAE(3.125、6.250、12.500、25.000、50.000μg·mL^(−1))组,除对照组外,其他各组均给予0.4-0.2 mmol·L^(−1)的O-P诱导细胞脂质沉积,诱导3 h后开始给药,干预24 h后,CCK-8法检测细胞活性,油红O染色观察细胞中脂滴形成情况,试剂盒法测定细胞中TG、TC、谷胱甘肽(GSH)、超氧化物歧化酶(SOD)及丙二醛(MDA)水平。结果确定造模方式为:0.4-0.2 mmol·L^(−1)的O-P作用HepG2细胞3 h后开始给药;10、15μmol·L^(−1)的辛伐他汀和3.125~100.000μg·mL^(−1)的MOLAE作用于HepG2细胞24、48 h后,不影响细胞活力。与模型组比较,不同浓度的MOLAE(3.125、6.250、12.500、25.000、50.000μg·mL^(−1))均能降低TG、TC、MDA水平(P<0.05、0.01),显著升高GSH、SOD水平(P<0.05、0.01)。油红O染色结果表明,辛伐他汀组和MOLAE组(12.5、25.0μg·mL^(−1))脂滴堆积现象均较模型组有明显改善。结论MOLAE能够降低O-P诱导的HepG2细胞中TG、TC水平,提高GSH、SOD水平和降低MDA水平,减少细胞中脂质堆积的现象。Objective To investigate the regulating effects of Moringa oleifera leaf aqueous extract(MOLAE)on lipid abnormality in human HepG2 cells.Methods Detect the effect of sodium oleate-sodium palmitate(O-P)on HepG2 cell viability using the CCK-8 method,the effect of O-P on cell lipid accumulation using oil red O staining,and the effect of O-P on cell triglyceride(TG)and total cholesterol(TC)levels using the kit method.Screen the concentration and time of O-P induced abnormal lipid metabolism model in HepG2 cells.The CCK-8 method was used to detect the effects of simvastatin and MOLAE on the viability of HepG2 cells,and to screen for safe concentrations of action.Establish control group,model group,and simvastatin(positive drug,15μmol·L^(−1))group and the MOLAE(3.125,6.250,12.500,25.000,50.000μg·L^(−1))group,except for the control group,were all given O-P of 0.4-0.2 mmol·L^(−1)to induce cell lipid deposition.After induction for 3 h,the drug was administered.After 24 hours of intervention,cell activity was detected using CCK-8 method,lipid droplet formation was observed using oil red O staining,and the levels of TG,TC,superoxide dismutase(SOD),malondialdehyde(MDA),and glutathione(GSH)in cells were measured using a kit method.Results The modeling method was determined as follows:Administration of O-P with a concentration of 0.4-0.2 mmol·L^(−1)was initiated on HepG2 cells for 3 h.Simvastatin with 10 and 15μmol·L^(−1)and MOLAE of 3.125—100.000μg·mL^(−1)did not affect cell viability after 24 and 48 hours of action on HepG2 cells.Compared with the model group,different concentrations of MOLAE(3.125,6.250,12.500,25.000,50.000μg·mL^(−1))can reduce the levels of TG,TC,and MDA(P<0.05,0.01),and significantly increase the levels of GSH and SOD(P<0.05,0.01).The oil red O staining results showed that the accumulation of lipid droplets in the simvastatin group and MOLAE(12.5,25.0μg·mL^(−1))group was significantly improved compared with model group.Conclusion MOLAE can reduce TG and TC levels in model g

关 键 词：辣木叶水提物 油酸钠-钠棕榈酸酯 HEPG2细胞 脂质堆积 调血脂 抗氧化 

分 类 号：R285.5[医药卫生—中药学]