基于宏基因组技术研究哈蟆油对大鼠肠道菌群的影响  

Effect of Oviductus Ranae on gut microbiota of rats using macrogenomic technology

作　　者：张文庆 谢地 辛梦茹 林嫚婷 张觅[1] 刘亮[1] 刘冬玲[3] 郭珊 胡汉昆[1] Wen-Qing ZHANG;Di XIE;Meng-Ru XIN;Man-Ting LIN;Mi ZHANG;Liang LIU;Dong-Ling LIU;Shan GUO;Han-Kun HU(Department of Pharmacy,Zhongnan Hospital of Wuhan University,Wuhan 430071,China;Department of Pharmacy,Fudan University Shanghai Cancer Center(Xiamen),Xiamen 361000,Fujian Province,China;Department of Pharmacy,Huangshi Central Hospital,Huangshi 435001,Hubei Province,China;Department of Biological Repositories,Zhongnan Hospital of Wuhan University,Wuhan 430071,China)

机构地区：[1]武汉大学中南医院药学部,武汉430071 [2]复旦大学附属肿瘤医院厦门医院药剂科,福建厦门361000 [3]黄石市中心医院药剂科,湖北黄石435001 [4]武汉大学中南医院生物样本库,武汉430071

出　　处：《药物流行病学杂志》2023年第9期1008-1016,共9页Chinese Journal of Pharmacoepidemiology

基　　金：湖北省科技创新人才及服务专项软科学研究项目(CXRK2022000184)。

摘　　要：目的基于宏基因组技术研究哈蟆油(OR)对大鼠肠道菌群的影响,用组学技术表征OR的功效。方法雄性SD大鼠随机分为对照组和OR组。OR组大鼠以200 mg·kg^(-1)剂量的OR匀浆每日灌胃,对照组以等体积生理盐水灌胃。14 d后,采集大鼠粪便进行宏基因组测序,对测序结果进行基因预测、丰度分析、物种注释及京都基因与基因组百科全书(KEGG)功能注释。结果在门水平上OR可以显著升高放线菌门相对丰度(P<0.05),在纲水平上OR可以显著升高放线菌纲相对丰度(P<0.05),在目水平上OR可以显著升高双歧杆菌目相对丰度(P<0.05)。两组大鼠肠道菌群在门和属水平上的β多样性存在差异。线性判别效应大小分析(LEfSe)筛选出两组间35种显著差异菌种。KEGG功能注释结果显示,在Level 1层级上注释到新陈代谢类通路的基因最多。组间差异分析表明,OR组注释到免疫系统、环境适应及癌症相关通路的相对丰度产生变化。结论改变大鼠肠道菌群相对丰度及多样性、调节脂质及氨基酸代谢通路可能是OR营养保健和治疗疾病的潜在机制。Objective To study the effect of Oviductus Ranae(OR)on gut microbiota of rats based on macrogenomic technology,and to characterize the efficacy of OR by histological techniques.Methods Male SD rats were randomly divided into the control group and the OR group.Rats in the OR group were gavaged daily with 200 mg·kg^(-1) dose of OR homogenate,while rats in the control group were gavaged with an equal volume of saline.After 14 days,rat feces were collected for macro-genome sequencing,and the sequencing results were subjected to gene prediction,abundance analysis,species annotation and Kyoto Encyclopedia of Genes and Genomes(KEGG)functional annotation.Results OR significantly increased the relative abundance of Actinobacteria at the phylum level and the class level(P<0.05),and Bifidobacteriales at the order level(P<0.05).There were differences in the Beta diversity of the intestinal flora of the two groups of rats at the phylum and genus levels.Linear discriminant analysis found 35 significantly different bacteria between two groups.The KEGG functional annotation results showed that most genes were annotated to metabolic pathways at the Level 1.The analysis of differences between groups showed that the OR group annotated the relative abundance of pathways related to the immune system,environmental adaptation and cancers changed.Conclusion Altering the relative abundance and diversity of rat gut microbiota and modulating lipid and amino acid metabolic pathways may be potential mechanisms for OR nutritional care and disease treatment.

关 键 词：哈蟆油 肠道菌群 宏基因组 新陈代谢 

分 类 号：R285.5[医药卫生—中药学]