粉蕉PAL家族基因的鉴定及其在逆境胁迫下的表达分析  

Identification of PAL family genes in Pisang Awak and their expression analysis under stress conditions

作　　者：曾坚 王舒婷 周洁薇 胡伟[2,3] 曾力旺 ZENG Jian;WANG Shu-ting;ZHOU Jie-wei;HU Wei;ZENG Li-wang(Guangdong Provincial Key Laboratory of Utilization and Conservation of Food and Medicinal Resources in Northern Region,Shaoguan University/Henry Fok College of Biology and Agriculture,Shaoguan University,Shaoguan 512005,China;Institute of Tropical Bioscience and Biotechnology,Chinese Academy of Tropical Agricultural Sciences,Haikou 571101,China;Institute of Scientific and Technical Information,Chinese Academy of Tropical Agricultural Sciences,Haikou 571101,China)

机构地区：[1]韶关学院广东省粤北食药资源利用与保护重点实验室/韶关学院英东生物与农业学院,广东韶关512005 [2]中国热带农业科学院热带生物技术研究所,海南海口571101 [3]中国热带农业科学院科技信息研究所,海南海口571101

出　　处：《江苏农业学报》2023年第6期1286-1293,共8页Jiangsu Journal of Agricultural Sciences

基　　金：广东省基础与应用基础研究基金项目(2023A1515010336、2021A1515011236);广东省普通高校重点领域专项(2022ZDZX4047);韶关学院重点项目(SZ2022KJ05);韶关学院博士启动项目(99000615);国家自然科学基金项目(31901537);国家级大学生创新创业训练计划项目(202310576009)。

摘　　要：苯丙氨酸解氨酶(PAL)在植物生长发育和对生物/非生物胁迫的响应中起着重要作用,因此有必要对香蕉中的PAL家族基因进行详细的研究。本研究从粉蕉基因组中鉴定出8个MaPAL基因,分别命名为MaPAL1~MaPAL8,在系统发育树中MaPAL6基因单独被分成一支。根据基因系统进化关系、蛋白质结构域和基因结构的分析,8个MaPAL基因属于PAL家族基因。转录组分析结果表明,MaPAL基因参与了粉蕉的发育、成熟以及对生物/非生物胁迫的响应。MaPAL4基因在所有果实发育和成熟阶段都表现出高表达。MaPAL1和MaPAL7基因可能对非生物胁迫有响应,MaPAL2和MaPAL8基因的表达在香蕉枯萎病菌4号生理小种(Foc4)侵染时被明显抑制,可能响应Foc4的侵染。和巴西蕉相比,MaPAL基因在粉蕉中被低温和渗透胁迫诱导的程度要高。本研究结果为进一步研究香蕉PAL家族基因的功能提供了基础,也为香蕉遗传改良提供了潜在基因资源。Phenylalanine ammonia-lyase(PAL)plays an important role in plant growth,development and response to biotic/abiotic stresses.Therefore,it is necessary to investigate PAL family genes in banana.In this study,eight MaPAL genes were identified from the Pisang Awak genome,which were named as MaPAL1-MaPAL8.The MaPAL6 gene was individually divided into one branch.The phylogenetic relationship,protein domain and gene structure analysis showed that eight MaPAL genes belonged to PAL family genes.Transcriptomic analysis showed that MaPAL gene was involved in the development,maturation and response to biotic/abiotic stresses.The MaPAL4 was highly expressed at fruit development and maturation stages.MaPAL1 and MaPAL7 might respond to abiotic stress.The expression of MaPAL2 and MaPAL8 was significantly inhibited during the infection of Foc4,which may respond to the infection of Foc4.Compared with Brazilian banana,MaPAL genes were highly induced by low temperature and osmotic stress in Pisang Awak.These results provide a basis for further study on the function of PAL family genes and potential genetic resources for banana genetic improvement.

关 键 词：粉蕉 PAL家族基因 非生物胁迫 生物胁迫 

分 类 号：S668.1[农业科学—果树学]