副猪嗜血杆菌血清7型crp基因缺失株的部分生物学特性  

Partial biological characteristics of Haemophilus parasuis serotype 7 crp gene deletion strain

作　　者：徐引弟[1] 蔡旭旺[2] 徐晓娟[2] 王治方[1] 张家庆[1] 朱文豪[1] 雷亚楠 张立宪[1] 李海利[1] 焦文强[1] 王克领[1] XU Yin-di;CAI Xu-wang;XU Xiao-juan;WANG Zhi-fang;ZHANG Jia-qing;ZHU Wen-hao;LEI Ya-nan;ZHANG Li-xian;LI Hai-li;JIAO Wen-qiang;WANG Ke-ling(Institute of Animal Husbandry and Veterinary Research,Henan Academy of Agricultural Sciences,Zhengzhou 450002,China;State Key Laboratory of Agricultural Microbiology,College of Veterinary Medicine,Huazhong Agricultural University,Wuhan 430070,China)

机构地区：[1]河南省农业科学院畜牧兽医研究所,河南郑州450002 [2]华中农业大学动物医学院农业微生物国家重点实验室,湖北武汉430070

出　　处：《江苏农业学报》2023年第6期1372-1379,共8页Jiangsu Journal of Agricultural Sciences

基　　金：国家重点研发计划项目(2021YFD1301205);河南省科技攻关项目(212102110378);河南省农业科学院自主创新项目(2022ZC43)。

摘　　要：副猪嗜血杆菌(HPS)是引起猪格拉瑟病(Glasser’s disease)的病原菌,给全球和中国规模化养猪业造成了重大经济损失。副猪嗜血杆菌血清型众多,其中血清7型是近年来分离比例越来越高的血清型,也是危害越来越严重的血清型。crp是最重要的系统调控因子之一,在细菌感染过程中对细菌适应环境变化起着至关重要的作用。为了筛选副猪嗜血杆菌血清7型crp基因缺失弱毒株,采用自然转化法构建了副猪嗜血杆菌血清7型临床分离株HPS7的crp基因缺失株,对HPS7及其crp基因缺失株的生长特性、生物膜形成、抗药性、毒力等进行了研究。结果表明,HPS7的crp基因缺失后,生长显著减慢,自凝速度减慢,生物膜形成能力明显减弱,对大部分抗生素的抗性降低,对豚鼠的毒力降低。以上结果说明crp基因对HPS7的生长、抗药性、毒力均有明显影响。本研究结果为筛选副猪嗜血杆菌血清7型弱毒株提供了参考。Haemophilus parasuis(HPS)is the pathogen that causing porcine Glasser’s disease,causing great economic losses to the large scale pig industry in the world and China.There are many serotypes of HPS,serotype 7 is a serotype with increasing proportion of isolation in recent years,and also a serotype with more and more serious harm.The crp is one of the most important systemic regulatory factors and plays a crucial role in adapting to environmental changes during bacterial infection.In order to screen HPS serotype 7 crp gene deletion attenvated strain,the crp gene deletion strain of HPS serotype 7 clinical isolate HPS7 was constructed by natural transformation method.Growth characteristics,biofilm formation,drug resistance and virulence of HPS7 and crp deletion strain were studied.The results showed that the growth speed was significantly reduced,autoagglutination speed was showed down,the ability of biofilm formation was obviously weakened,resistance to most antibiotics was reduced,and the virulence to guinea pig was decreased after crp was deleted in HPS7.The results showed that crp gene had significant influence on growth,drug resistance and virulence of HPS7.The results of this study provide a reference for the screening of HPS serotype 7 attenuated strains.

关 键 词：副猪嗜血杆菌血清7型 crp基因缺失株 生长特性 抗药性 毒力 

分 类 号：S858.31[农业科学—临床兽医学]