一株高效降解血液蛋白的枯草芽孢杆菌NWMCC0137全基因组测序及分析  

Whole genome sequencing and analysis of Bacillus subtilis NWMCC0137, an efficient blood protein degrading strain

作　　者：刘高强 李新鹏 刘文钊 周魏 彭乔烽 周雪雁[1,2] 默罕默德·索布瑞·塔克瑞夫 丁功涛 LIU Gao-qiang;LI Xin-peng;LIU Wen-zhao;ZHOU Wei;PENG Qiao-feng;ZHOU Xue-yan;MOHAMMED Sobri-takriff;DING Gong-tao(China-Malaysia National Joint Laboratory,Biomedical Research Center,Northwest Minzu University,Lanzhou 730030,China;College of Life Sciences and Engineering,Northwest Minzu University,Lanzhou 730030,China;College of Chemical Engineering,Northwest Minzu University,Lanzhou 730030,China;Department of Chemical and Process Engineering,Faculty of Engineering and Built Environment University Kebangsaan Malaysia,Bangi 43600,Malaysia)

机构地区：[1]西北民族大学生物医学研究中心中国-马来西亚国家联合实验室,甘肃兰州730030 [2]西北民族大学生命科学与工程学院,甘肃兰州730030 [3]西北民族大学化工学院,甘肃兰州730030 [4]马来西亚国立大学工程与建筑环境学院化工系,雪兰莪州万宜43600

出　　处：《江苏农业学报》2023年第7期1460-1471,共12页Jiangsu Journal of Agricultural Sciences

基　　金：“科技助力经济2020”重点专项(SQ2020YFF0405583);西北民族大学中央高校基本科研业务经费项目(31920220023);西北民族大学中央高校基本科研业务费资助项目(31920180122)。

摘　　要：NWMCC0137是从屠宰场下水道污泥中分离获得的1株高效分解血液蛋白并具有良好生物防控特性的枯草芽孢杆菌(Bacillus subtilis)。为探究枯草芽孢杆菌NWMCC0137(B.subtilis NWMCC0137)高效水解血液蛋白机制及挖掘次级代谢产物编码基因资源,利用DNBSEQ与PacBio测序平台对其进行全基因组测序并进行序列组装和基因预测、功能注释、比较基因组分析。结果表明B.subtilis NWMCC0137的基因组长度为4215585 bp,G+C含量为44.23%;编码基因总长度为3711885 bp,编码4384个基因,编码序列占整个基因组长度的88.05%,非编码RNA中包含85个tRNA基因。在KEGG数据库中B.subtilis NWMCC0137基因组被注释到7种胞外蛋白酶编码基因,其中与丝氨酸蛋白酶相关的编码基因有8个。通过antiSMASH预测发现,菌株NWMCC0137基因组中包含7种与已知次生代谢产物合成基因簇相似度为100%的基因簇,包括产孢致死因子、聚酮类化合物、丰原素、枯草芽孢杆菌素168、儿茶酚型嗜铁素、芽孢杆菌素、杆菌溶素合成基因簇。进化分析结果表明,NWMCC0137为枯草芽孢杆菌,并且与枯草芽孢杆菌168具有很高的共线性。B.subtilis NWMCC0137基因组序列已提交至GenBank数据库,登录号为CP103066。本研究从基因层面揭示了菌株NWMCC0137的遗传信息,为该菌株应用于屠宰场血液蛋白降解及生物防控提供了参考。NWMCC0137 is a Bacillus subtilis strain isolated from sewage sludge in slaughterhouses,which efficiently decomposes blood proteins and has good biological control characteristics.In order to explore the mechanism of efficient hydrolysis of blood protein by Bacillus subtilis NWMCC0137 and explore the gene resources encoded by secondary metabolites,DNBSEQ and PacBio sequencing platforms were used to sequence the whole genome,and sequence assembly,gene prediction,functional annotation and comparative genome analysis were carried out.The results showed that the genome length of Bacillus subtilis NWMCC0137 was 4215585 bp,and the G+C content was 44.23%.The total length of the coding gene was 3711885 bp,encoding 4384 genes,accounting for 88.05%of the entire genome length.The non-coding RNA contained 85 tRNA genes.In KEGG database,Bacillus subtilis NWMCC0137 genome was annotated to seven extracellular protease coding genes,including eight coding genes related to serine protease.According to the prediction of antiSMASH,the genome of strain NWMCC0137 contained seven gene clusters with 100%similarity to the known secondary metabolite synthesis gene clusters,including sporulation killing factor,bacillaene,fengycin,sublancin 168,bacillibactin,subtilosin A and bacilysin gene clusters.The evolutionary analysis results indicated that NWMCC0137 was a Bacillus subtilis and had high collinearity with Bacillus subtilis 168.The genome sequence of Bacillus subtilis NWMCC0137 had been submitted to the GenBank database with accession number CP103066.This study revealed the genetic information of strain NWMCC0137 at the genetic level,providing a reference for its application in degrading blood proteins and biological prevention and control.

关 键 词：枯草芽孢杆菌 全基因组 基因功能注释 蛋白酶 

分 类 号：Q934.124[生物学—微生物学]