远缘原生质体融合菌株的构建及生防作用研究  

Constructing a Distant Protoplast Fusion Strain of Rhodopseudomonas palustris and Bacillus thuringiensis to Biocontrol Ginger Root-Knot Nematodes

作　　者：康霄文 杜晓华 王东伟 程菊娥[2] 王忠勇[2] 孙书娥[2] 苏品[2] 刘勇[2] KANG Xiao-wen;DU Xiao-hua;WANG Dong-wei;CHENG Ju-e;WANG Zhong-yong;SUN Shu-e;SU Pin;LIU Yong(Asset Management Center of Hunan Academy of Agricultural Sciences,Changsha 410125,PRC;Hunan Institute of Plant Protection,Hunan Academy of Agricultural Sciences,Changsha 410125,PRC)

机构地区：[1]湖南省农业科学院资产管理中心,湖南长沙410125 [2]湖南省植物保护研究所,湖南省农业科学院,湖南长沙410125

出　　处：《湖南农业科学》2023年第10期43-49,共7页Hunan Agricultural Sciences

基　　金：湖南省自然科学基金青年基金(2022JJ40234);杂交水稻全国重点实验室自主研究项目(2023MS03);湖南省农业科技创新资金(2023CX18,2022CX1,2022CX84-18);长沙市自然科学基金(kq2202338)。

摘　　要：研究基于沼泽红假单胞菌PSB-06促生和增强寄主抗病性,苏云金芽孢杆菌KY-1抗逆性强等优点,采用原生质体融合技术获得这2种菌株的融合菌株,测定了融合菌株对生姜根结线虫的防治效果,以期得到综合亲本优良性状的新型高效生防菌株。结果表明:融合菌株显著降低了土壤根结线虫的数量,收获期相对防效达73.68%;融合菌株处理显著提高了生姜的农艺性状、产量和品质,单株姜球数(13.80个)、姜球宽度(33.47 cm)和单株产量(1.29 kg)均优于空白对照;同时提高了生姜收获期叶片的总叶绿素含量、POD和SOD活性。综合来看,沼泽红假单胞菌和苏云金芽孢杆菌融合菌株的促生及生防潜力较亲本沼泽红假单胞菌有所提高,为微生物菌种改良提供了依据。Microbial protoplast fusion breeding is an important direction of microbial strain improvement.Based on the advantages of Rhodopseudomonas palustris PSB-06 for promoting growth and enhancing host resistance,and strong stress resistance of Bacillus thuringiensis KY-1,their fusion strain was obtained by protoplast fusion technology,whose control effect on ginger root-knot nematode disease was tested.The results showed that the fusion strain treatment significantly reduced the number of soil root-knot nematodes,and the disease control effect reached 73.68%in the harvest period.Meanwhile,it significantly improved agronomic traits,yield and quality of ginger;the number of ginger bulb per plant(13.80),ginger bulb width(33.47 cm)and the yield per plant(1.29 kg)all surpassed the blank control;at the same time,the total chlorophyll content,and POD and SOD activities of ginger in the harvest period were increased.In conclusion,the fusion strain of Rhodopseudomonas palustris and Bacillus thuringiensis has produced much stronger growth promotion and biocontrol effects than the parent strain PSB-06,which may provide theoretical and practical support for the improvement of microbial strains.

关 键 词：沼泽红假单胞菌 苏云金芽孢杆菌 原生质体融合 生姜 根结线虫 

分 类 号：Q813.2[生物学—生物工程]