中性脂含量高的微拟球藻藻株的快速筛选  

Rapid Screening of Neutral Lipid Content High Nannochloropsis oceanica Strains

作　　者：陈小凡 王路路[1] 肖腾飞 朱葆华[1] 李赟[1] 潘克厚[1,2] Chen Xiaofan;Wang Lulu;Xiao Tengfei;Zhu Baohua;Li Yun;Pan Kehou(Key Laboratory of Mariculture(Ocean University of China),Ministry of Education,Qingdao 266003,China;Laboratory for Marine Fisheries Science and Food Production Processes,Laoshan Laboratory,Qingdao 266237,China)

机构地区：[1]海水养殖教育部重点实验室(中国海洋大学),山东青岛266003 [2]崂山实验室海洋渔业科学与食物产出过程功能实验室,山东青岛266237

出　　处：《中国海洋大学学报（自然科学版）》2023年第12期32-39,共8页Periodical of Ocean University of China

基　　金：国家自然科学基金项目(41976118)资助。

摘　　要：微拟球藻(Nannochloropsis oceanica)生长速度快、油脂含量高,已成为生产生物柴油的理想材料。为获得中性脂产率高的藻株,本研究从染色时间和染色浓度两个方面优化了尼罗红染色测定中性脂含量的方法,对不同育种方法得到的75株微拟球藻藻株的产油能力进行了评价。采用优化后的染色条件(尼罗红浓度1 mg/mL,染色时间10 min),测定了第9天微藻的中性脂相对含量及对应的生物量,获得藻株中性脂的相对产率。研究表明,与原始藻株相比,088号转基因藻株相对产率为252.16%,甲基磺酸乙酯(EMS)诱变藻株101号相对产率为259.02%,亚硝基胍(NTG)诱变藻株231号相对产率为323.84%。本研究获得了3株中性脂产率高的微拟球藻藻株,这3株藻株有望应用于生物柴油的生产,研究结果可为高油脂含量微藻的快速筛选提供参考。Nannochloropsis oceanica has become an ideal material for biodiesel production due to its fast growth rate and high lipid content.In order to obtain high neutral lipid yielding strains,we optimized the Nile red staining method in staining time and dye concentration for determining the neutral lipid content,and evaluating the lipid producing ability of 75 strains of N.oceanica obtained with different breeding methods.Under the optimized staining conditions(Nile red,1 mg/mL;staining time,10 min),we assayed the relative content of neutral lipid and biomass growth on day 9 and calculated the relative yield of neutral lipids.We found that the relative yield of a transgenic strain,088,was 252.16%of the original strain,the relative yield of an ethyl methane sulfonate(EMS)mutant,101,was 259.02%of the original,and the relative yield of a N-methyl-N′-nitro-N-nitrosoguanidine(NTG)mutant,231,was 323.84%of the original.Three strains with high neutral lipid yields wereapplicable for producing biofuel.Our findings provided a reference for the rapid screening of microalgal strains with high lipid content.

关 键 词：微拟球藻 中性脂 尼罗红 藻种选育 

分 类 号：S968.42[农业科学—水产养殖] O657.3[农业科学—水产科学]