脑红蛋白在神经细胞铁死亡中的作用研究  

Research on the Effects of Neuroglobin on Ferroptosis in the Nerve Cells

作　　者：高雯晶 莫晨 冯巍 潘新民[1] 秦豪杰[1] Wenjin Gao;Chen Mo;Wei Feng;Xinmin Pan;Haojie Qin(1School of Basic Medicine and Forensic Medicine,Henan University of Science and Technology,Luoyang,Henan,China)

机构地区：[1]河南科技大学基础医学与法医学院,河南洛阳471000

出　　处：《Chinese Medicine and Natural Products》2023年第3期133-142,共10页中医学报（英文）

基　　金：funded by Henan Science and Technology Research Program Project(222102310398).

摘　　要：目的:探讨脑红蛋白在神经细胞铁死亡中的作用。方法:通过构建小鼠海马神经元细胞HT22的脑红蛋白(neuro-globin,NGB)敲低模型,分别检测Normal组、Erastin组、NGBsiRNA组和NGBsiRNA+Erastin组,细胞增殖活性、铁离子含量、丙二酵含量、超氧化物含量、活性氧含量等铁死亡相关指标,及核因子E2相关因子2(nuclear factor E2-related factor 2,Nrf2)表达的变化情况。结果:Norma组相比,Erastin组、NGBsiRNA组和NGBsiRNA+Erastin组细胞增殖活性和Nrf2表达量均显著降低,铁离子含量、丙二酵含量、超氧化物含量、活性氧含量等铁死亡相关指标均显著升高,NGBsiRNA+Erastin组与Erastin组、NGBsiRNA组相比差异有统计学意义(p<0.05)。结论:敲低NGB可以增强铁死亡诱导剂Eras-tin的作用,NGB可能通过Nrf2调控细胞铁死亡过程,该研究为中医药临床治疗神经系统疾病提供参考。Objectives The objective of this article was to explore the effects of neuroglobin(NGB)on ferroptosis in the nerve cells.Methods The NGB knockdown model of HT22 cells was constructed,and the ferroptosis-related indexes of cell proliferation activity,contents of iron ion,malondialdehyde(MDA),superoxide and reactive oxygen,and the changes of nuclear factor E2-related factor 2(Nrf2)expression were examined in the normal group,erastin group,NGB siRNA group,and NGB siRNAtErastin group,respectively.Results Compared with the normal group,cell proliferation activity and Nrf2 expression were significantly lower in the erastin group,NGB siRNA group,and NGB siRNAterastin group,and ferroptosis-related indexes such as iron ion content,MDA content,superoxide content,and reactive oxygen species content were significantly reduced,and the difference between NGB siRNAterastin group,erastin group,and NGB siRNA group was statistically significant(p<0.05).Conclusion Knockdown of NGB in cells enhances the action of the ferroptosis inducer erastin,and NGB may regulate the cellular ferroptosis process through Nrf2.This research may provide references for the clinical treatment of nervous system disease with Chinese medicine.

关 键 词：脑红蛋白 HT22细胞 铁死亡 SIRNA 神经细胞 

分 类 号：R285[医药卫生—中药学]