Sequencing methods and functional decoding of mRNA modifications  

作　　者：Kai Li Jinying Peng Chengqi Yi 

机构地区：[1]State Key Laboratory of Protein and Plant Gene Research,School of Life Sciences,Peking University,Beijing 100871,China [2]Academy for Advanced Interdisciplinary Studies,Peking University,Beijing,100871,China [3]Peking-Tsinghua Center for Life Sciences,Peking University,Beijing,China [4]Department of Chemical Biology and Synthetic and Functional Biomolecules Center,College of Chemistry and Molecular Engineering,Peking University,Beijing,100871,China

出　　处：《Fundamental Research》2023年第5期738-748,共11页自然科学基础研究（英文版）

基　　金：the Ministry of Science and Technology of China(2019YFA0110902,2019YFA0802201)。

摘　　要：More than 160 types of post-transcriptional RNA modifications have been reported;there is substantial variation in modification type,abundance,site,and function across species,tissues,and RNA type.The recent development of high-throughput detection technology has enabled identification of diverse dynamic and reversible RNA modifications,including N6,2′-O-dimethyladenosine(m6Am),N1-methyladenosine(m1A),5-methylcytosine(m5C),N6-methyladenosine(m6A),pseudouridine(Ψ),and inosine(I).In this review,we focus on eukaryotic mRNA modifications.We summarize their biogenesis,regulatory mechanisms,and biological functions,as well as highthroughput methods for detection of mRNA modifications.We also discuss challenges that must be addressed in mRNA modification research.

关 键 词：RNA modification N6 2′-O-dimethyladenosine(m6Am) N1-methyladenosine(m1A) 5-Methylcytosine(m5C) N6-methyladenosine(m6A) Pseudouridine(Ψ) Inosine(I) 

分 类 号：G322[文化科学]