代谢物衍生的非酶促翻译后修饰研究进展  

Advance in Metabolite-mediated Non-enzymatic Post-translational Modifications

作　　者：董媛媛 鲍秋雨 王德祥 温宏涛 杨行超 皖宁 叶慧[1] DONG Yuan-yuan;BAO Qiu-yu;WANG De-xiang;WEN Hong-tao;YANG Xing-chao;WAN Ning;YE Hui(Key Laboratory of Drug Metabolism and Pharmacokinetics,China Pharmaceutical University,Nanjing 210009,China)

机构地区：[1]中国药科大学药物代谢动力学重点实验室,南京210009

出　　处：《中国生物工程杂志》2023年第10期43-51,共9页China Biotechnology

基　　金：国家自然科学基金(82173783);江苏省自然科学基金杰出青年基金(BK20220088)资助项目。

摘　　要：代谢物在细胞生命周期中具有重要作用,包括为细胞生命活动提供能量及合成原料、参与生物信号转导等。已有研究表明,活性代谢物可通过体内自发化学反应共价修饰蛋白,调控蛋白质结构和功能,进而影响细胞生命活动的各个环节。近年来,发展迅速的蛋白质组学技术能够捕获这类非酶促修饰的靶蛋白,继而阐明代谢物对靶蛋白功能的调控机制。由活性代谢物产生的非酶促共价修饰,分为可逆和不可逆两种类型。详细介绍代谢物衍生的非酶促翻译后修饰的形成机理,并列举蛋白质组学技术如何鉴定这类修饰进而揭示代谢调控机制的经典研究案例。Endogenous small molecules play vital roles in the cell lifespan,such as supplying the energy and substrates for biological processes and mediating cellular communication.Previous studies have shown that some endogenous reactive molecules can non-enzymatically modify proteins via spontaneous chemical reactions,thereby regulating protein structure and functions and participating in various cellular processes.Recently,the rapid development of proteomics technology for capturing non-enzymatically modified target proteins contributes to elucidating the function of the modified target protein.This review focuses on reversible and irreversible non-enzymatic covalent modifications(NECMs)produced by active metabolites,respectively,and introduces the formation mechanism of NECMs and the application of proteomics in the discovery of new post-translational modifications(PTMs)and the elucidation of metabolites-regulated protein functions.

关 键 词：活性代谢物 非酶促共价修饰 蛋白组学技术 

分 类 号：Q816[生物学—生物工程]